AOAC SPIFAN Stakeholder Panel Meeting Book (March 14, 2019)

Responses to Comment (PF cont.)

However, taurine in dairy products and infant formulas was presented as free taurine originally, I didn’t find relevant information about whether it bonds to other components in the dairy products and infant formulas under manufacture process.

Additional Research On Total vs. Free Taurine We reached out to one of the participants in the validation of 999.12 in regard to free versus total taurine. She had the following to say: • At the time there was a distinction between free taurine and total taurine. Free taurine was all that was tested in infant formulas as the majority for label claims was added via a premix. In pet food and other matrices in order to determine the amount of taurine present in the matrix hydrolysis was required to analyze the total taurine. My understanding is that it was bound to other peptides or proteins as was treated similarly to other matrices tested for total amino acids.