AOAC SPIFAN Stakeholder Panel Meeting Book (March 14, 2019)
First results ஜ-galactosidase (Action: FrieslandCampina)
- K-LOLAC enzyme from Megazyme kit (ஜ-galactosidase) - First screening performed: not only lactose but also GOS hydrolyzed
Blank lactose/GOS
Enzyme-treated GOS
- Alternative option: cellobiose dehydrogenase (CDH)
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Other open actions
• Selective resin for lactose scavenging in advance of the analysis (Action: WUR/FrieslandCampina)
• Ethanol precipitation of lactose (Action: FrieslandCampina)
Reduction Lactose (%)
Lactose content (%) GOS content (%) Recovery GOS (%)
60 % E-OH 70 % E-OH 80 % E-OH
10,0
3,4 3,3 2,3
112,2 109,3 75,6
71,3 81,5 90,5
6,5 3,3
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