AOAC SPIFAN Stakeholder Panel Meeting Book (March 14, 2019)

First results ஜ-galactosidase (Action: FrieslandCampina)

- K-LOLAC enzyme from Megazyme kit (ஜ-galactosidase) - First screening performed: not only lactose but also GOS hydrolyzed

Blank lactose/GOS

Enzyme-treated GOS

- Alternative option: cellobiose dehydrogenase (CDH)

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Other open actions

• Selective resin for lactose scavenging in advance of the analysis (Action: WUR/FrieslandCampina)

• Ethanol precipitation of lactose (Action: FrieslandCampina)

Reduction Lactose (%)

Lactose content (%) GOS content (%) Recovery GOS (%)

60 % E-OH 70 % E-OH 80 % E-OH

10,0

3,4 3,3 2,3

112,2 109,3 75,6

71,3 81,5 90,5

6,5 3,3

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