AOAC SPIFAN Stakeholder Program Meeting Book (August 28, 2021)

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Direct methods

Principle Detects GC or LC -TAG profile

Pros

Cons

Information on TAG profile

- 0D\ QHHG 06 RU +506 IRU GHWHFWLRQ DQG TXDQWLILFDWLRQ ,QVWUXPHQWDWLRQ FRVWV KLJK - Need of pure expensive standards. - Need of mathematical models to convert TAG profile into sn -2 fatty acid content. - *& PHWKRG LGHQWLILHV 7$*V DFFRUGLQJ WR FDUERQ QXPEHU &1 QRW VHOHFWLYH

-sn -2 fatty acids

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-sn -2 fatty acids Qualitative and quantitative

- Instrumentation cost high. - The applicability is limited in complex mixtures. - No information about the identity of individual TAG species.

information on the positional isomerism of fatty acids present in TAGs

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Challenges

X +DYH D PHWKRG WKDW LV DOVR VXLWDEOH IRU RLOV DQG IDW KDYLQJ PHOWLQJ SRLQW °C. X +DYH D PHWKRG WKDW LV DOVR VXLWDEOH IRU RLOV DQG IDWV KDYLQJ KLJK DPRXQW RI IDWW\ DFLGV ” & DQG • & X ,Q &KLQD QXWULWLRQDO FODLPV DUH RQ 232 FRQWHQW DQG QRW RQ sn -2 palmitate. A method addressing only the quantification of sn -2 fatty acids will not allow the quantification of OPO.

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