AOAC SPIFAN WPC ERP-Final Action Review (Nov. 2017)

2016.15 (Nov. 2017) - WPC-31 MLT-Final Action Review

FOR ERP USE ONLY DO NOT DISTRIBUTE

Figure 2016.15D: eGram of Typical Infant Formula

Low MW whey

Casein

High MW whey

References: A1973A Application Information of Protein Characterization, Beckman Coulter; A5190AD Application Guide of PA 800 plus Pharmaceutical Analysis System, Beckman Coulter.

Appendix: Data processing using 32 Karat and excel 1. Stop automatic Caesar integration 1.1. Log into 32Karat Enterprise

1.2. On the main screen, right click on “SDS MW”, choose Configure > Instrument 1.3. In the new window (Instrument Configuration), click the “Configure” button 1.4. Click “Options” 1.5. Remove the check mark in front of Caesar Integration 1.6. Click “OK”

2. Integrate the eGram

2.1. Open the eGram (File> Data > open)

PDA - 220nm SX2 SX2

PDA - 220nm Blank blank

0.006

0.006

Migration Time

0.004

0.004

14.433 14.692

16.517 16.708

0.002

0.002

AU

AU

0.000

0.000

-0.002

-0.002

0.0

2.5

5.0

7.5

10.0

12.5

15.0

17.5

20.0

22.5

25.0

27.5

30.0

Minutes