AOAC SPIFAN WPC ERP-Final Action Review (Nov. 2017)

2016.15 (Nov. 2017) - WPC-31 Publications-Final Action Review

FOR ERP USE ONLY DO NOT DISTRIBUTE

2.4 Perform a manual integration of the larger whey proteins relative to casein. For this integration, choose the auto integration stop point as the start point of the manual integration and align the baseline with blank. Example:

Note: the manual integration should include all peaks after the start of manual integration until the end of the run, as long as peaks are detected.

3. Data calculation 3.1. .Edit the custom report to have a table containing only migration time and area 3.2. Choose the custom report from the report menu 3.3. Copy the table to an Excel sheet 3.4. Move the data from the internal standard out of the table (in red below) 3.5. Separately sum the lower molecular weight whey proteins, the caseins and the higher molecular weight whey proteins (if multiple peaks are detected):

12.3 38938

M igration Tim e

A rea 6049 2777

0.010

0.010

12

PDA - 220nm SX3

PDA - 220nm Blank

12.5 12.6

Migration Time Area Percent

2435 13 13927

0.008

0.008

14.167 9.304

13.3 12843 13.6 10240 13.9 38437 14.2 53699 14.4 103273 Sum LM W W hey 243680

13.925 6.660

15.792 7.088

16.183 12.595

0.006

0.006

16.392 16.169

14.442 17.894

AU

AU

0.004

0.004

12.975 2.413

12.000 1.048

13.283 2.225

13.633 1.774

12.483 0.481 12.625 0.422

17.575 1.489

15.8 40909 16.2 72691 16.4 93315 17.6 8592 17.8 24258 Sum Casein 239765

17.750 4.203

22.417 5.470

20.108 4.017

0.002

0.002

12.275 6.747

0.000

0.000

12

14

16

18

20

24

22

26

20.1 23181 22.4 31567 Sum H M W W hey 54748

Minutes

Casein

LowMWwhey

High MWwhey

3.6. Calculate the whey protein content according to equations (1) and (2), taking into account the 1.4 correction factor for whey proteins.