AOAC SPSFAM ERP for SELECTED FOOD ALLERGENS
AOAC SPDS ERP - Set 3 Review Form 2 Name Linda Monaci E-mail linda.monaci@ispa.cnr.it Organization CNR-ISPA Title of Method
Detection and Quantitation of Selected Food Allergens by LCMS/MS
AOAC Candidate Method Number (e.g. ALN-01) Applicable SMPR
ALL-01
yes
Summary:
The method described is a LC-MS/MS method for the simultaneous detection of multiple allergens basing on the detection of unique peptide markers for each allergenic food namely egg, milk, peanut and hazelnut along the same LC-MS run. The method has been run on different triple quadrupole MS instrument by building an acquisition method monitoring at least two transitions for each peptide marker targeted. The sensitivity, specificity and applicability of the method has been also investigated in the different food commodities analysed.
I.
Review of Method Only
1. Does the applicability of the method support the applicability of the SMPR? If not, please explain what is missing.
Yes
2. Does the analytical
Despite the analytical technique that in principle is expected to meet the SMPR requirements, there is still an incorrect way of calculating MQL and MDL. This arises from an erroneous noise estimation and consequently an erroneous calculation of the resulting S/N. Despite the correct approach the authors stick with the use of a
technique(s) used in the method meet the SMPR? If not, please specify how it
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