AOAC SPSFAM ERP for SELECTED FOOD ALLERGENS

differs from what is stated in the SMPR.

software under AB SCIEX company for the estimation of S/N. According to my experience these kind of software are very much optimistic and will not allow comparability of results obtained on different machines commercialized by different companies. The SMPR reports that the estimation of s0 done in the proper way should lead to reliably calculate the final MQL and MDL. Yes for many of them. Still some problems in the correct calculation of MDL and MQL and in the calculation of the recovery that should be done on 7 independent analysis for each concentration level tested (referred to spiked or incurred blank samples) (and not 3 independent analysis for each concentration level as done in this work)

3. Are the definitions specified in the SMPR used and applied appropriately in the method? If no, please indicate how the terms are used. 4. Does the method, as written, contain all appropriate precautions and warnings related to the method’s reagents, components, instrumentation, or method steps that may be hazardous? If no, please suggest wording or option(s). 1. Are the definitions specified in the SMPR used and applied appropriately in the supporting documentation (manuscripts, method studies, etc…)? If not, please explain the differences and if the method is impacted by the difference. II.

Yes

Review of Supporting Information

Yes although some confusion appears in the manuscript especially in the chromatograms shown in figures 7, 8, 9, 10. It is never specified if the chromtagramas shown at 0 and 10 ppm levels refer to spiked or incurred food samples….this could make a big difference.