AOAC SPSFAM ERP for SELECTED FOOD ALLERGENS

statements in the method? 2. Is there information

demonstrating that the method system suitability tests and controls as specified in the SMPR worked appropriately and as expected? If no, please specify. 3. Based on the supporting information, is the method written clearly and concisely? If no, please specify the needed revisions. 4. Based on the supporting information, what are the pros/strengths of the method? 5. Based on the supporting information, what are the cons/weaknesses of the method? 6. Any general comments about the method?

yes

yes

the pros is the potential of the MS/MS based method to detect along one run several allergens such as egg, milk, hazelnut, peanut with possible inclusion of other nuts according to some preliminary data shown. Also another advantage is the sharp protocol required for sample preparation. sensitivity of the method should be better investigated and also recovery of the food matrices depending on the type of inclusion done whether spiked or incurred. The method has been definitely improved and enriched with additional details and info also thanks to other experiments carried out according to what raised by the reviewers. I would approve the method only if calculation of recovery is properly done (also detailing clearly if it refers to the spiked or incurred food samples; and if it is done according to what reported in the guidance namely n=7 independent analysis for each concentration level). Most importantly I would require to calculate in a correct way the MDL and MQL that is a very