AOAC SPSFAM ERP for SELECTED FOOD ALLERGENS

2. Is there information demonstrating that the method system suitability tests and controls as specified in the SMPR worked appropriately and as expected? If no, please specify. 3. Based on the supporting information, is the method written clearly and concisely? If no, please specify the needed revisions. 4. Based on the supporting information, what are the pros/strengths of the method? 5. Based on the supporting information, what are the cons/weaknesses of the method?

Yes

Yes

Comprehensive evaluation of the specified marker peptides in selected matrices in selected samples. The concept evaluated is a strong point of the method.

The method has been demonstrated to be applicable to selected matrices. The contents of unique marker peptide is different in even in the same matrix type like cookie thus method may requires separate calibration curve for every sample type even for those matrices where it has been demonstrated to be working. The method has not been demonstrated for its performance in different types of the matrices in scope like Cookie etc. It's a very good concept but how practical to apply it in field not known. The methods accuracy not established using other reference materials (which have not used to calibrate the method) and by analysis using other established methods for the same samples.

6. Any general comments about the method?

Do you recommend this method be adopted as a First Action and published in the Official Methods of Analysis of AOAC INTERNATIONAL? Please specify rationale. V. Final Recommendation

The method may be adopted as a First Action for only matrices for which its performance has been demonstrated satisfactory in the submitted report.