AOAC SPSFAM ERP for SELECTED FOOD ALLERGENS

- The peptides are, however, shown to be from the correct proteins in Appendix 1.

Milk: - The authors do indicate two peptides from Bos d 9 (alpha-s1-casein). However, the other two peptides given for milk are from different proteins. Milk.Protein_2.Peptide_A is from Bos d 5 (beta- lactoglobulin) and Milk.Protein_2.Peptide_B is from Bos d 11 (beta-casein). Despite the name given to the peptides, they are not from the same protein. For milk, the authors therefore have two peptides for one protein and only one peptide for two additional proteins. In the case of the beta- lactoglobulin and beta-casein peptides, there is only one quantifier peptide and one qualifier peptide between these two proteins. only been conducted for one peptide (two transitions) for each commodity-matrix combination. What happens when the quantifier ion is detected but the qualifier ion(s) is/are not? It is unclear from the description of the preparation of the incurred/spiked foods whether all of the target allergenic foods (milk, egg, peanut, and hazelnut) were incurred/spiked into the same batch of the food matrix (e.g. cookie) or whether each allergenic food was incurred into separate batches of the food matrices. The authors also do not consistently and clearly state all units. Due to the differences in potential differences, all units must be complete and specific, - Again, the peptides are, shown to be from the correct proteins in Appendix 1. In addition, while the authors state that a total of eight MRM transitions for each allergen commodity are used in the method, validation seems to have