AOAC SPSFAM ERP for SELECTED FOOD ALLERGENS

AOAC SPSFAM ERP REVIEW FORM - MARCH 13, 2017

Submission Date

2017-02-28 12:44:08

Name

Tomasz Tuzimski

E-mail

tomasz.tuzimski@umlub.pl

Organization

Medical University in Lublin, Poland

Title of Method

Detection and Quantitation of Selected Food Allergens using LC-MS/MS (Revision 2)

AOAC Candidate Method Number (e.g. ALN-01)

ALL-01 (Revision 2)

Applicable SMPR

2016.002

The proposed method entitled ‘Detection and Quantitation of Selected Food Allergens using LC-MS/MS (Revision 2)’ described by Lee Sun New, Andre Schreiber and Hua- Fen Liu is applicable for the detection and quantitation of egg, milk, peanut, and hazelnut food allergens in finished food products and ingredients by LC-MS/MS. The method uses triple quadrupole mass spectrometry and selective Multiple Reaction Monitoring (MRM) of characteristic transitions of precursor ions to fragment ions of multiple proteins and peptides to uniquely identify each allergen. Characteristic signature peptides were chosen for each alergen, and MRM transitions for each signature peptide were determined based on their uniqueness compared to background proteins and their sensitivity of detection. For each allergen multiple unique peptides were chosen from unique proteins, and two MRM transitions per peptide were chosen. The method performance requirements were met for the detection of egg, milk, peanut and hazelnut in a number of food matrices. The method can be easily extended to the detection of other allergens, including soy and other tree nuts (almonds, Brazil nut, cashew, pine nut, pistachio, pecan, and walnut). The method was verified in-house and across two other different laboratories. YES: The applicability of the method is adequate to the applicability of the SMPR. The target commodity analytical range of 10 ‒ 1000 ppm for all matrices with recoveries (refer to Definitions) between 60 ‒ 120% and RSDr of less than 20% were achieved for all allergen commodities in the food matrices required for AOAC SMPR 2016.002.

I. General Summary:

2. Does the analytical technique(s) used in the method meet the SMPR? If not, please specify how it differs from what is stated in the SMPR. 3. Are the definitions specified in the SMPR used and applied appropriately in the method? If no, please indicate how the terms are used. the method support the applicability of the SMPR? If not, please explain what is missing. II. Review of the Method Only: 1 Does the applicability of

YES: The analytical techniques in the method are adequate and meet the SMPR.

YES: Definitions, which are specified in the SMPR, were listed in the description, also were applied appropriately in the method.