AOAC SPSFAM ERP for SELECTED FOOD ALLERGENS

2. Is there information demonstrating that the method meets the SMPR Method Performance Requirements using the Reference Materials stated in the SMPR? If not, then specify what is missing and how this impacts demonstration of performance of the method. method performs within the SMPR Method Performance REquirements table specifications for all analytes in the SMPR applicability statement? If not, please specify what is missing and whether or not the method's applicability should be modified. additional steps in the evaluation of the method that indicated the need for any additional precautionary statements in the method? 2. Is there information demonstrating that the method system suitability tests and controls as specified in the SMPR worked appropriately and as expected? If no, please specify. 3. Based on the supporting information, is the method written clearly and concisely? If no, please specify the needed revisions. 4. Based on the supporting information, what are the pros/strengths of the method? 3. Is there information demonstrating that the 1. Based on the supporting information, were there any

YES

YES

IV. General Submission Package:

YES, Internal standards should be added prior to the extraction to understand the actual recoveries of analytes in sample matrices.

YES

YES however SRM results must be reported.

1.Most confirmative method as it is MRM method with eight transitions per allergen.

2. Used SRMs and labeled standards

3. Linearity studies conducted as per SMPR.