AOAC SPSFAM PAC ERP

I. Summary of the Method

Proanthocyanidins are extracted with 70% acetone in an ultrasonic bath for 15 min (X3) then filtered and centrifuged. The solution was then loaded on a Sephadex LH-20 column and eluted with ethanol, then ethanol/methanol (1:1), then 80% acetone and the 80% acetone eluent was reserved to isolate the PACs from the other phenolics. The eluent was evaporated and solubilized in methanol. The methanol solution was then spotted on a stainless steel target followed by the addition of DHB. A specific technique was used to assure that the sample and matrix would co-crystallize evenly (no coffee ring effect). Calibrants including procyanidin A2 mixed with procyanidin B2 were mixed by mass and the spectra were collected. The overlapping isotopic distribution patterns were studied and understood and a deconvolution model was developed allowing for the determination of the ratio of A-type to B-type linkages.

II. Review of the Method Only:

II. Review of the Method Only 1. Does the applicability of the method support the applicability of the SMPR? If not, please explain what is missing. 2. Does the analytical technique(s) used in the method meet the SMPR? If not, please specify how it differs from what is stated in the SMPR. 3. Are the definitions specified in the SMPR used and applied appropriately in the method? If no, please indicate how the terms are used. 4. Does the method, as written, contain all appropriate precautions and warnings related to the method's reagents, components, instrumentation, or method steps that may be hazardous? If no, please suggest wording or option(s).

The method investigated frozen CPC powder alone and mixed with apple products. The applicability statement calls for one or more of the following: fruit, juice, beverage, dried cranberry fruit, cranberry sauce, ingredients, and/or dietary supplements. It might be nice to see how this performs in a different matrix, however, technically it meets the requirements of the SMPR and I was impressed by the addition of testing a material with no A-type linkages.

Yes.

Yes.

I did not notice safety precautions, however, there are very few that are likely to be necessary.

III. Review of Information in Support of the Method