AOAC SPSFAM PAC ERP

2. Does the method contain system suitability tests or controls as specified by the SMPR? If not, please indicate if there is a need for such tests or controls and which ones. 3. Is there information demonstrating that the method system suitability tests and controls as specified in the SMPR worked appropriately and as expected? If no, please specify. 4. Based on the supporting information, is the method written clearly and concisely? If no, please specify the needed revisions. 5. Based on the supporting information, what are the pros/strengths of the method?

YES: Yes, there are.

YES: There is a piece of information demonstrating that the method system suitability tests and control as specified in the SMPR worked appropriately and expected.

The method is well described and substantively prepared. The concepts, analyses, and methodology are adequately developed. The method proposed is well integrated and well-reasoned.

In my opinion the pros/strengths of the method can be following: 1. The internal standard used for quantification analyse of analyte should, as little as possible, differ from the analyte determined. Use of the c-PAC standard to quantify PAC content in cranberry samples resulted in values that were higher than those determined by procyanidin A2. Increased accuracy is critical for estimating PAC content in relationship to research on authenticity, efficacy, and bioactivity (also especially in designing clinical trials for determination of putative health benefits). 2. The total of seventeen (n=17), six-point standard reference curves, were generated by the operators (n=3) using both c-PAC and ProA2 reference standards. The cons/weakness of the method may be connect with: Specificity is one of most important analytical parameter. In my opinion, Authors may/should showed matrix effect: that - there were are or no - interferences from the matrices. The following aspects of the method (summary, presentation of the topic, definition of research objectives, presentation of research methodology and detailed presentation of method) are described in a satisfactory manner.

6. Based on the supporting information, what are the cons/weaknesses of the method?

7. Any general comments about the method?

Recommendation for the Method

V. Final Recommendation Do you recommend this method be adopted as a First Action and published in the Official Methods of Analysis of AOAC INTERNATIONAL? Please specify rationale.

In my opinion, the AOAC Candidate Method (#PAC-002) described by Christian Krueger et al. can be futher refined and next adopted in its modified form as a First Action and recommended for publication in the Official Methods of Analysis of AOAC INTERNATIONAL.