AOAC SPSFAM PAC ERP

II. Review of the Method Only 1. Does the applicability of the method support the applicability of the SMPR? If not, please explain what is missing. 2. Does the analytical technique(s) used in the method meet the SMPR? If not, please specify how it differs from what is stated in the SMPR. 3. Are the definitions specified in the SMPR used and applied appropriately in the method? If no, please indicate how the terms are used. 4. Does the method, as written, contain all appropriate precautions and warnings related to the method's reagents, components, instrumentation, or method steps that may be hazardous? If no, please suggest wording or option(s). III. Review of Supporting Information 1. Are the definitions specified in the SMPR used and applied appropriately in the supporting documentation (manuscripts, method studies, etc...)? If not, please explain the differences and if the method is impacted by the difference.

Yes. According to the publication, this method is applicable to quantitation of total proanthocyanidins (Procyanidin A2 and Procyanidin B2) after thiolysis of procyanidin B2. It was applied to three different matrices: dried cranberries, cranberry juice and and dietary supplement.

The method used is suitable to meet the SMPR. No specific requirements were made regarding the method used. There is some information that is not easily described within the methodology to understand how thiolysis is performed on the authentic samples.

Yes, the definitions are applied appropriately, with the exception that it would be desirable to collect repeatability data on authentic samples as opposed to spiked samples.

No - hazards are not specified in the method.

III. Review of Information in Support of the Method

Yes, with the exception of repeatability (authentic samples would be desirable). It is difficult to understand the RSDr in relation to total procyanidin calculations in authentic samples.