AOAC SPSFAM PAC ERP

AOAC DECEMBER 2017 ERPs - METHOD REVIEW FORM

Submission Date

2017-12-11 10:06:52

AOAC Expert Review Panel: Method Review

Reviewer Information Required to validate your review.

Name

Kate Rimmer

E-mail

catherine.rimmer@nist.gov

Organization

NIST

Remember, the main purpose of your review is to ensure the method conforms to the applicable SMPR. View and download SMPRs here:

SPDS SMPRs SPSFAM SMPRs

Method Review

Title of Method

Determination of proanthocyanidins in cranberry-containing products by normal phase high performance liquid chromatography: single laboratory validation

AOAC Candidate Method Number (e.g. ALN-01)

PAC-005

Applicable SMPR

2017.003

I. Summary of the Method

I. Summary of the Method

The method abstract says that the method is to quantify A-type PACs in cranberry products. The first step of the method isolates the total PAC fraction by loading an aqueous extract on a sephadex LH-20 column, eluted to remove the anthocyanins (as monitored by absorbance at 520 nm) then the PAC are eluted with acetone:water (7:3). Samples and calibrants were then injected on a diol column, eluted with a gradient of acetonitrile:acetic acid (98:2) and methanol:water:acetic acid (95:3:2). Fluorescence detection was used with an excitation wavelength at 230 nm and emission at 321 nm.

II. Review of the Method Only: