AOACSPIFANMethods-2017Awards

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218 G olay & M oulin : J ournal of AOAC I nternational V ol . 99, N o . 1, 2016

acids by the simple addition of individual fatty acids results corresponding to each class or group (expressed in grams fatty acids (FA) per 100 g product):

This calculation can be performed only when the fat content is determined with an appropriate and validated fat extraction method. Do not use the declared fat value for the expression of fatty acids on finished products due to possible imprecision between fat labeled and fat extracted. ( 4 ) Sum of class or group of fatty acids. —Calculate the mass fraction of all fatty acids included in a group or in a class of fatty

∑

= 3 FA gFA g gFA g gFA g i i i + + ( / 100 / 100 / 100 ) 1 2

Table 2012.13. Stoichiometric factors for converting FAMEs to fatty acids

Position of unsaturation (terminal methyl carbon)

FAME mol. wt, g/mol

(FA) a

Chain length

Configuration Group Abbrev.

FA mol. wt, g/mol TAG mol. wt, g/mol

S i

C4:0 C6:0 C8:0

SFA SFA SFA SFA SFA SFA

102.1 130.2 158.3 186.3 214.4 242.4 240.4 256.4 254.4 270.5 268.5 284.5 282.5 298.5 296.5 296.5 294.5 294.5 294.5 292.5 292.5 292.5 326.6 324.6 322.5 320.5 320.5 318.5 316.5 354.6 352.6 350.6 342.5 382.7 380.7

88.1

302.4 386.5 470.7 554.9 639.0 723.2 717.1 765.3 759.2 807.3 801.3 849.4 843.4 891.5 885.5 885.5 879.4 879.4 879.4 873.4 873.4 873.4 975.7 969.6 963.6 957.5 957.5 951.5 945.4

0.863 0.892 0.911 0.925 0.935 0.942 0.942 0.945 0.945 0.948 0.948 0.951 0.950 0.953 0.953 0.953 0.952 0.952 0.952 0.952 0.952 0.952 0.957 0.957 0.957 0.956 0.956 0.956 0.956 0.960 0.960 0.960 0.959 0.963 0.963

116.2 144.2 172.3 200.3 228.4 226.4 242.4 240.4 256.4 254.4 270.5 268.4 284.5 282.5 282.5 280.5 280.5 280.5 278.4 278.4 278.4 312.5 310.5 308.5 306.5 306.5 304.5 302.5 340.6 338.6 336.6 328.5 368.7 366.6

C10:0 C12:0 C14:0 C14:1 C15:0 C15:1 C16:0 C16:1 C17:0 C17:1 C18:0

MUFA b

ω-5 (or n -5)

Cis

SFA

ω-5 (or n -5)

MUFA

Cis

SFA

ω-7 (or n -7)

MUFA

Cis

SFA

ω-7 (or n -7)

MUFA

Cis

SFA

Trans c

C18:1 TFA

C18:1

ω-9 (or n -9)

MUFA

Cis

Trans c

C18.2 TFA

LA d

C18:2

ω-6 (or n -6) ω-6 (or n -6) ω-6 (or n -6)

PUFA PUFA PUFA

Cis

C18:2 CLA

Cis / trans

CLA

C18:3

Cis

Trans c

C18:3 TFA

ALA e

C18:3 C20:0 C20:1 C20:2 C20:3 C20:3 C20:4 C20:5 C22:0 C22:1 C22:2 C22:6 C24:0 C24:1

ω-3 (or n -3)

PUFA

Cis

SFA

ω-9 (or n -9) ω-6 (or n -6) ω-6 (or n -6) ω-3 (or n -3) ω-6 (or n -6) ω-3 (or n -3) ω-9 (or n -9) ω-6 (or n -6) ω-3 (or n -3)

MUFA PUFA PUFA PUFA PUFA PUFA

Cis

Cis

Cis

Cis

ARA f EPA g

Cis

Cis

SFA

1059.9 1053.8 1047.8 1023.6 1144.0 1137.9

MUFA PUFA

Cis

Cis

PUFA DHA h

Cis

SFA

ω-9 (or n -9)

MUFA

Cis

a  S i (FA) = Stoichiometric factor to convert FAME i b  MUFA = Monounsaturated fatty acid. c  Does not include TFAs in MUFA and PUFA sums. d  LA = Linoleic acid.

to FAi.

e  ALA = α-Linolenic acid. f  ARA = Arachidonic acid.

g  EPA = Ecosapentaenoic acid. h  DHA = Docosahexaenoic acid.