AOACSPIFANMethods-2017Awards

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1588  H aselberger & J acobs : J ournal of AOAC I nternational V ol . 99, N o . 6, 2016

Table 3. Data for evaluating LOQ Sample type 0.03% FOS spike in 9% sucrose solution a Infant formula powder with FOS/GOS c a  No additional matrix, solution in water. b  Triplicates on each of 3 days. c  Material from SPIFAN sample kit. d  Duplicates on each of 6 days.

No. of replicates Mean (g/100 g, RTF)

SD r

RSD r

, % SD IP

RSD IP

, %

9 b

0.029

0.0018

6.12

0.0021

7.42

12 d

0.036

0.0008

2.14

0.0012

3.29

and generating results for the Abbott in-house samples listed in Table 2016.06F .

Conclusions

Performance metrics from the validation work indicate that the method conforms to the range, LOQ, precision, and recovery requirements established in SMPR 2014.002. In addition, specificity appears to be adequate, as evidenced by the failure to detect any apparent fructan in the unfortified samples that were analyzed. Common carbohydrate ingredients, GOS and various maltodextrins, although producing more complicated chromatograms for interpretation, do not generally create undue difficulty for assignment of CFs in the qualitative analysis. We conclude that the method is suitable for determination of inulin- type fructans in infant formulas and pediatric/adult nutritional products.

References

 (1)  Official Methods of Analysis (2016) 20th Ed., AOAC INTERNATIONAL, Rockville, MD, SMPR 2014.002. www. eoma.aoac.org  (2) Joye, D., & Hoebregs H. (2000) J. AOAC Int . 83 , 1020–1026  (3) De Leenheer, L., & Hoebregs, H. (1994) Starch – Stärke 46 , 193–196  (4) Durgnat, J., & Martinez, C. (1997) Seminars in Food Analysis 2 , 85–97  (5) L’homme, C., Peschet, J.L., Puigserver, A., & Biagini, A. (2001) J. Chromatogr. A 920 , 291–297  (6) Hoebregs, H. (1997) J. AOAC Int . 80 , 1029–1037  (7) McCleary, B.V., Murphy, A., & Mugford, D.C. (2000) J. AOAC Int . 83 , 356–364  (8) Stöber, P., Bénet, S., & Hischenhuber, C. (2004) J. Agric. Food Chem . 52 , 2137–2146  (9) Steegmans, M., Iliaens, S., & Hoebregs, H. (2004) J. AOAC Int. 87 , 1200–1207 (10) Cuany, D., Bénet, T., & Austin, S. (2010) J. AOAC Int. 93 , 202–212

Acknowledgments

The authors wish to thank Stephanie (Williams) Neu, Bao Kou Thao, Ashely (Johnson) Bonavia, David Ellingson, Brent Rozema, and Vanessa Spencer from Covance Laboratories (Madison, WI) and Ding Yi from Abbott Nutrition, Singapore, for their efforts in setting up themethodology in their laboratories