AOACSPIFANMethods-2017Awards

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12 H ostetler : J ournal of AOAC I nternational V ol . 100, N o . 3, 2017

Table 10. Precision data for β-carotene in SPIFAN matrixes and select additional matrixes a

cis -BC, μg/100 g b RSD r

trans -BC, μg/100 g RSD r

Total BC, μg/100 g RSD r

Formula

Code

, % RSD iR

, %

, % RSD iR

, %

, % RSD iR

, %

n

NA c

00847RF00 3

0.3

0.7

Child Formula Powder, Placebo Infant Elemental Powder, Placebo AN High-Protein RTF, Placebo e SRM 1849a Powder IF Milk-Based Partially Hydrolyzed Powder IF Soy-Based Partially Hydrolyzed Powder Toddler Formula Milk-Based Powder IF Milk-Based Powder Child Formula Powder IF Elemental Powder IF with FOS/GOS Powder g IF Milk-Based RTF, Placebo f AN Powder

0.6

2.6

NA 0.9

1.8

NA

< d

00796RF 3

NA

NA

<

NA NA

<

NA NA

00821RF00 3

<

NA

NA

0.8

11.2

NA 0.8

11.2

NA

AN High-Fat RTF, Placebo 00820RF00 3

<

NA 3.3 NA 0.2

NA NA NA NA

0.4 0.4

47.4

NA 0.6 NA 0.9

48.5

NA NA

EV4H2Q 3 CLC10-B 3 410057652Z 3

0.5

2.2

2.6

<

<

NA NA

<

NA NA

0.8

1.0

2.0

NA 1.8

1.2

NA

410057651Z 3

0.6

1.8

NA

0.6

6.7

NA 1.3

3.9

NA

4052755861 12

6.5

1.0

2.4

7.4

0.7

2.9

13.9

0.8

2.2

4044755861 12 00859RF00 3 00866RF00 3 00795RF 12 50350017W1 12 K16NTAV 39

4.4

2.1 NA

2.7 NA NA 0.7 6.3 2.7 2.3 NA 2.7 NA NA NA

10.1

1.3 5.9 2.5 0.7 3.0 0.5 2.0 4.8 1.7 7.7

2.7

14.4

1.5 5.9 5.4 0.5 2.3 0.6 2.2 4.6 1.6 5.9

2.4 NA NA 0.6 3.8 1.7 1.9 NA 2.4 NA NA

<

0.3 0.4

NA 0.3 NA 0.7

0.3 7.3 1.3 6.0

15.3

0.3 5.1 1.1 2.6 6.9 1.6 NA NA NA

15.3

0.7 4.6 2.2

22.7

3.4

4.8

13.2

19.2

IF Milk-Based Powder (control) IF Soy-Based Powder IF Milk-Based RTF AN High-Protein RTF

E10NWZC 12 EV4H2R 3

6.5 0.6

11.0

1.8

17.5

0.4

NA 1.0

00730RF00 12 265

534

2.3

799

AN High-Fat RTF

00729RF00 3

< < <

0.3 0.5

NA 0.4 NA 0.5

— h

AN High-Lutein Powder

3

18.3

18.3

Chocolate Pediatric Nutritional RTF

— 3

<

NA NA

<

NA NA

IF RTF

— 3

9.1

0.3

NA

16.4

0.4

NA 25.5

0.4

NA

a  For powder samples, results were calculated using a factor of 25 g powder/225 g reconstituted weight. b  BC = β-Carotene. c  NA = Not applicable. RSDr not calculated for concentrations

accuracy was assessed by spike and recovery of all- trans -lutein and β-carotene in various matrixes. Spiking was done with either FloraGlo 5% lutein (DSM Nutritional Products, Basel, Switzerland), which was first dispersed in water and added to the sample in place of water, or with diluted standards added to the ISTD. Spiked and unspiked samples were measured in triplicate. Tables 11–17 show that recovery from spiked samples ranged from 92.3 to 105.5% for lutein and from 100.1 to 107.5% for β-carotene. The SMPR calls for d  < = Below the LOQ. e  AN = Adult Nutritional. f  IF = Infant Formula. g  FOS/GOS = Fructooligosaccharide/galactooligosaccharide. h  — = Sample was sourced locally and was not part of the SPIFAN kit. Table 11. Spike and recovery data for NIST SRM 1849a a Analyte Native level, μg/100 g

90–110% recovery from spiked samples, and all data met this requirement.

Conclusions

The data presented here were reviewed by the AOAC Expert Review Panel on SPIFAN Nutrient Methods at the AOAC INTERNATIONAL Annual Meeting in Dallas, TX, held in September 2016. The panel found that the data met the criteria

Spike, μg/100 g Recovery, % RSD, % Spike, μg/100 g Recovery, % RSD, %

ND b

Lutein

1.4 1.3

105.5 107.5

0.8 0.9

13.8 13.6

99.6

0.2 0.3

β-Carotene

0.2

103.2

a  Samples were spiked with standards at the internal standard step of sample preparation. b  ND = Not detected.