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H ostetler : J ournal of AOAC I nternational V ol . 100, N o . 3, 2017  13

Table 12. Spike and recovery data for NIST SRM 1849a a

Native level, μg/100 g

Spike, μg/100 g Recovery, % RSD, %

Spike, μg/100 g

Spike, μg/100 g Recovery, % RSD, %

Analyte

Recovery, % RSD, %

ND b

Lutein

34.8

92.3

0.8

68.3

94.0

1.9

135.3

97.6

1.6

a  Samples were spiked with FloraGlo 5% lutein at the powder reconstitution step of sample preparation. b  ND = Not detected. Table 13. Spike and recovery data for AN High-Protein RTF, Placebo a

Native level, μg/100 g

Spike, μg/100 g Recovery, % RSD, %

Spike, μg/100 g Recovery, % RSD, % Spike, μg/100 g Recovery, % RSD, %

Analyte

Lutein

3.3

62.7

96.7

0.8

148.8

100.1

0.3

297.6

101.0

0.3

a  Samples were spiked with FloraGlo 5% lutein at the initial sample dilution step.

Table 14. Spike and recovery data for AN High-Protein RTF, Placebo a

Native level, μg/100 g

Spike, μg/100 g Recovery, % RSD, % Spike, μg/100 g Recovery, % RSD, % Spike, μg/100 g Recovery, % RSD, %

Analyte

Lutein

3.3

486.4

101.1

0.5

708.8

101.2

0.5

1425.3

100.7

0.2

a  Samples were spiked with FloraGlo 5% lutein at the initial sample dilution step.

Table 15. Spike and recovery data for Child Formula Powder, Placebo a Analyte

Native level, μg/100 g Spike, μg/100 g Recovery, % RSD, % Spike, μg/100 g Recovery, % RSD, %

Lutein

0.1 0.6

5.5 5.3

101.8 107.2

0.2 0.2

16.8 16.2

100.8 104.2

0.3 0.3

β-Carotene

a  Samples were spiked with standards at the internal standard step of sample preparation.

Table 16. Spike and recovery data for Infant Elemental Powder, Placebo a Analyte Native level, μg/100 g Spike, μg/100 g

Recovery, % RSD, % Spike, μg/100 g Recovery, % RSD, %

ND b

Lutein

5.5 5.3

103.7 104.1

0.2 0.2

16.8 16.2

101.9 103.5

0.6 0.6

β-Carotene

0.1

a  Samples were spiked with standards at the internal standard step of sample preparation. b  ND = Not detected.

Table 17. Spike and recovery data for Infant Formula RTF a Analyte Native level, μg/100 g

Spike, μg/100 g Recovery, % RSD, % Spike, μg/100 g Recovery, % RSD, %

Lutein

3.9

6.2 5.9

98.7

0.4 0.9

18.4 17.7

98.6

0.1 0.6

β-Carotene

16.4

100.6

100.1

a  Samples were spiked with standards at the internal standard step of sample preparation.

for specific carotenoids, (lutein and β-carotene) as stated in SMPR 2014.014, and recommended the method’s approval as an AOAC First Action method. It is codified as Official Method 2016.13 .

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Acknowledgments

I thank Shang-Jing Pan and Douglas Gordon of Abbott Nutrition (Columbus, OH) for reviewing the method and providing feedback, and Steve Tennyson and Scott Christiansen of Perrigo Nutritionals (Georgia, VT) for reviewing the method and validation data.

References

(1) Canfield, L.M., Clandinin, M.T., Davies, D.P., Fernandez, M.C., Jackson, J., Hawkes, J., Goldman, W.J., Pramuk, K., Reyes, H., Sablan, B., Sonobe, T., & Bo, X. (2003) Eur. J. Nutr. 42 , 133–141