AOACSPIFANMethods-2017Awards

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1394 Jaudzems: J ournal of AOAC I nternational V ol. 98, N o. 5, 2015 The analytical range for SPIFAN samples was found to be between 1.4 and 1060 mg/100 g reconstituted product or RTF. The LOQ was estimated as 1.4 mg/100 g. Results of precision studies for chloride expressed on reconstituted products are presented in Table 2015.08A . RSD r ranged from 0.03 to 1.60% in the range from 20 to 167 mg/100 g RTF, and RSD R ranged Conclusions

All results compared to SMPR values are shown in Table  2015.08C . All results were within the target values defined in AOAC SMPR 2014.015.

from 0.09 to 2.77% over the same range, thus fulfilling the ≤ 2 and 4% performance requirements, respectively. All samples met the intermediate reproducibility (RSD R ) performance requirement except for one RTF sample with high fat content, which gave a precision value of 10.09%. This RTF sample was problematic due to heterogeneity and separation since it was being used beyond its recommended shelf life. Accuracy was proven by analyzing SRM 1849a in duplicate on 6 different days and comparing the overall mean to the SRM informational value (not certified; Table 2015.08B ). The overall mean was 68.5 mg/100 g for the reconstituted product with an RSD R of 1.59%, equivalent to a recovery of 97.2%. Spike recovery was performed on 15 different SPIFAN products (powder and liquid infant formula and adult nutritionals). The reconstituted powders and RTF products were spiked at two different levels (50 and 100% of native chloride content) and analyzed in duplicate on 6 different days (Table 2015.08B ). Spike recovery (99–104%) was within the tolerance (95–105%) defined in the SMPR.

The data presented in this paper were submitted to the AOAC ERP for review at the AOAC INTERNATIONAL Mid-Year Meeting held on March 17, 2015. The ERP determined that the data presented were in accordance with SMPR 2014.015 approved by SPIFAN, and the method was granted First Action status. (1) AOAC SMPR 2014.015, J. AOAC Int . 98 , 1079. http://dx.doi. org/10.5740/jaoacint.SMPR2014.016 (2) Sullivan, D. (2012) J. AOAC Int . 95 , 278–297. http://dx.doi.org/ 10.5740/jaoacint.Sullivan_Intro (3) Official Methods of Analysis (2012) 19th Ed., AOAC INTERNATIONAL, Rockville, MD, Appendix L: AOAC Recommended Guidelines for Stakeholder Panel on Infant Formula and Adult Nutritionals (SPIFAN) Single-Laboratory Validation, http://www.eoma.aoac.org References