Allergens ERP Book, Sept 17

AOAC SPDS ERP REVIEW: MAIN FORM

Submission Date

2017-09-12 17:49:30

Name

SNEH BHANDARI

E-mail

SNEH.BHANDARI@MXNS.COM

Organization

Merieux NutriSciences

Title of Method

Detection and Quantitation of Selected Food Allergens using LC-MS/MS

AOAC Candidate Method Number (e.g. ALN-01)

ALL-01

Applicable SMPR

2016.002

Summary:

A LC-MS/MS based method for the detection and quantitation of whole egg (egg white and egg yolk), whole milk, peanut butter, and hazelnut allergens in 10 food matrices was developed and evaluated in a single-lab validation. A LC-MS/MS analysis of the signature tryptic peptides of the 4 allegens was performed using multiple reaction monitoring. The matrices tried included cookies, ice cream, breakfast cereals , salad dressing, wine, milk/dark chocolate

1. Does the applicability of the method support the applicability of the SMPR? If not, please explain what is missing. 2. Does the analytical technique(s) used in the method meet the SMPR? If not, please specify how it differs from what is stated in the SMPR. 3. Are the definitions specified in the SMPR used and applied appropriately in the method? If no, please indicate how the terms are used. 4. Does the method, as written, contain all appropriate precautions and warnings related to the method's reagents, components, instrumentation, or method steps that may be hazardous? If no, please suggest wording or option(s).

Yes

Yes

Yes

Yes