Amino-01 (July 2018)

Amino-01 (JULY 2018) 180124 AA SPIFAN SLV Appendix J

FOR ERP USE ONLY DO NOT DISTRIBUTE

Single Laboratory Validation Report for Total Amino Acids by UHPLC-UV in Infant Formulas and Adult Nutritionals – APPENDIX J

Additional data: results from NIST inter-laboratory study for SRM 1869

Results from NIST inter-laboratory study for SRM 1869. Consensus result was determined from 14 participating labs. Final NIST CoA to follow upon release of SRM in 2018.

SRM 1869 (draft CoA)

avg. of triplicate (mg/100g)

Consensus mean NIST Interlab study

Amino acid

% Recovery

His Tau Ser Arg Gly Asp Glu Thr Ala Pro Lys Tyr Cys Met

0.364 0.037 0.793 0.527 0.319 1.357 2.937 0.679 0.544

0.357 0.038 0.768 0.556 0.316 1.298 2.909 0.685 0.532 1.253 1.116 0.599 0.162 0.471 0.859 0.768 1.394 0.669

102%

97%

103%

95%

101% 105% 101%

99%

102%

1.24

99%

1.215 0.656

109% 110%

0.16

99% 94%

0.441

Val

0.86

100% 100% 100% 101%

Ile

0.771 1.391 0.674

Leu Phe

Jaudzems, Guthrie, Lahrichi, and Fuerer – January 2018

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