Amino Acids Working Group Meeting Book (July 3, 2018)

Challenges Total AA analysis requires hydrolysis and analysis steps. Each introduces its own complications. • Complex Collection of Molecular Structures • No Common Structural Features Compatible with Direct, Sensitive, Detection • Asn and Gln converted to Asp and Glu during Acid Hydrolysis resulting Asx and Glx determination. • No single method can release all of the amino acids from protein - Destruction of Cys(or CSSC) and Trp during Acid Hydrolysis*

Existing Methods - General

• Ion Exchange/Ninhydrin PCR/Colorimetric Detection

• Pre-column Derivatization/HPLC