Amino Acids Working Group Meeting Book (July 3, 2018)

AOAC SPIFAN AMINO ACIDS WORKING GROUP Table 2. Method Performance Requirements a

Amino acids

ALA

ARG

ASN c

ASP/ASX d

CYS d

GLN c

GLU/GLX d

GLY

HIS

ILE

LEU

Analytical range b

100- 1520

40-650

55-420

60-190

9-1290

10-155

0.4-750

18-3110

30-290

24-380

19-795

Limit of quantitaion b

40

55

60

9

10

0.4

18

30

24

19

100

Recovery

RSDr

RSDR

Amino acids

LYS

MET

PHE

PRO

SER

TAU

THR

TRP

TYR

VAL

Analytical range b

0.4- 2500

72-1220

15-620

40-700

40-1440

40-865

1-45

16-770

13-615

60-915

Limit of quantitation b

72

15

40

40

40

1

16

≤0.4

13

60

±10% ±8% ±5%

Recovery

≤4% ≤3% ≤2%

RSDr

RSDR ≤6% ≤4% ≤3% a Concentrations apply to (a) ‘ready-to-feed’ liquids ‘as is’ ; (b) reconstituted powders (25 g into 200 g water); and (c) liquid concentrates diluted by weight using water. b mg/100g reconstituted final product. c Free only. d ASX = sum of ASN +ASP, CYS = sum of CSSC + CYS, GLX = sum of GLN + GLU.

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