Anli Gao-Stats Member

AOAC INTERNATIONAL

Methods Committee on Statistics Member Review

November 18, 2014

AOAC INTERNATIONAL 2275 Research Blvd., Suite 300 Rockville, MD, 20850

UNITED STATES dboyd@aoac.org 301.924.7077 x126

AOAC INTERNATIONAL Demographic Information Form

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Z A 5 3 g ; = 7 5 3 a [ ? X 5 A = 7 a \ A 8 ? X 5 A = 7 a 8 ? 2 < g 6 = 7 5 3 T < 4 9 = 6 ; ? g a T < 4 9 = 7 5 3 S A X = A 4 4 ? = A X a 8 ? ? 4 3 5 ; 4 B 5 ? 4 5 k T m S Y l [ ^ m S _ k [ ^ m S _ l e 8 7 ; 8 ? 5 ; 4 e 4 X ? 4 4 k e _ l Y 5 6 ; 4 ? „ 6 e 4 X ? 4 4 k Y Z l ‰ C 5 3 = ; g Z 6 6 C ? 5 A 7 4 ˆ ‰ C 5 3 = ; g T 8 A ; ? 8 3 k ‰ Z U ‰ T l U 5 > 4 ; g k U Z F S ^ l U 7 = 4 A ; = > = 7 ˆ ^ 4 7 < A = 7 5 3 k U T ^ S T l ‰ C 5 3 = ; g Z 6 6 C ? 5 A 7 4 ˆ ‰ C 5 3 = ; g T 8 A ; ? 8 3 k ‰ Z U ‰ T l U 5 > 4 ; g k U Z F S ^ l U 7 = 4 A ; = > = 7 ˆ ^ 4 7 < A = 7 5 3 k U T ^ S T l T < 4 9 = 6 ; ? g L [ ; < 4 ? ; < 5 A Z A 5 3 g ; = 7 5 3 k T T m S Y l Y = 7 ? 8 D = 8 3 8 X g  T < 4 9 = 6 ; ? g k Y T m S Y l [ ; < 4 ? k [ ^ m l Y 5 A 5 X 4 ? = 5 3 k Y ] d _ l T 8 A 6 C 3 ; 5 A ; k T ] U ` ^ l d ? 5 B C 5 ; 4 U ; C B 4 A ; k d _ Z e l \ A B C 6 ; ? g L e = 6 ; ? = D C ; 8 ? k e \ U ^ _ l \ A B C 6 ; ? g L \ A 6 ; ? C 9 4 A ; Y 5 A C > 5 7 ; C ? 4 ? k \ ] U ^ _ l \ A B C 6 ; ? g L _ 5 • Y 5 ; 4 ? = 5 3 6 U C : : 3 = 4 ? k _ Z – Y ^ l \ A B C 6 ; ? g L _ 4 ; 5 = 3 4 ? k _ ^ Z \ ` l \ A B C 6 ; ? g L ^ 4 6 ; Ž = ; T 8 9 : 5 A g k ^ U ^ Ž ^ l _ 4 ; = ? 4 B k _ S ^ _ e l

(Please complete other side)

È " & † & ) o p q r s t p q  € q  w v z { p v r p | } ~  € q  ‡  v y  ” y q t } ‡ } y ~ z t q q € { v t p É Ê É o  Ë Ì Í Î Ë É Ì  Ê Ë É Ï \ A V 8 3 V 4 9 4 A ; = A 5 T 8 9 9 C A = ; g k T [ Y l Y 4 ; < 8 B 6 — 5 3 = B 5 ; = 8 A 2 ? 8 7 4 6 6 k Y — 2 l h ' Ð 1 Œ 1 Ð h $ n o p q r s t p q  € q  w v z { p v r p | } ~ p  ‡ q x  € t v r v x  t q u { v t p É Ê É o  Ë Ì Í Î Ë É Ì  Ê Ë É Ï ‚ T 8 3 3 5 D 8 ? 5 ; 8 ? 8 A 5 Y 4 ; < 8 B 6 U ; C B g k T Y U l S B = ; 8 ? = 5 3 ƒ 8 5 ? B Y 4 9 D 4 ? k S ƒ Y l d 4 A 4 ? 5 3 _ 4 > 4 ? 4 4 k d _ l Y 4 ; < 8 B U ; C B g e = ? 4 7 ; 8 ? k Y U e l [ > > = 7 = 5 3 Y 4 ; < 8 B 6 ƒ 8 5 ? B Y 4 9 D 4 ? k [ Y ƒ l U 5 > 4 ; g Z B V = 6 8 ? k U Z l U ; 5 ; = 6 ; = 7 6 Z B V = 6 8 ? k U ^ Z ^ l " * $ ! " * " & & & " " * ( n & * n $ & o p q r s  y y t p q  € q  w }  q Ò x q € t v w q { p v r p  x x y | Z X ? = 7 C 3 ; C ? 5 3 F 4 4 B 6 a \ A 7 3 C B = A X Y 4 B = 7 5 ; 4 B k Z F S S l Z 3 3 4 ? X 4 A 6 k Z ` S _ l Z A ; = D = 8 ; = 7 _ 4 6 = B C 4 6 k Z ] _ U l Z X ? = 7 C 3 ; C ? 5 3 2 4 6 ; = 7 = B 4 F 8 ? 9 C 3 5 ; = 8 A 6 k Z 2 S U l Z X ? = 7 C 3 ; C ? 5 3 — 4 ; 4 ? = A 5 ? g ^ 8 G = 7 8 3 8 X g k Z — S ^ l ƒ 8 ; 5 A = 7 5 3 6 k ƒ [ ^ Z l T 8 9 : C ; 4 ? ^ 4 7 < A 8 3 8 X g ˆ U g 6 ; 4 9 6 k T [ Y 2 l e ? C X 5 A B e 4 V = 7 4 _ 4 3 5 ; 4 B Y = 7 ? 8 D = 8 3 8 X g k e e S — l m 8 C 6 4 < 8 3 B T < 4 9 = 7 5 3 6 k e m [ f l e = 4 ; 5 ? g U C : : 3 4 9 4 A ; 6 k e U f 2 l S A V = ? 8 A 9 4 A ; 5 3 Y = 7 ? 8 D = 8 3 8 X g k S Y \ T l F 8 8 B Z B B = ; = V 4 6 ˆ F 3 5 V 8 ? 6 ˆ U : = 7 4 6 k F Z e e l F 8 8 B T 8 9 : 8 6 = ; = 8 A 8 ? Z B C 3 ; 4 ? 5 ; = 8 A k F T [ Y l F 8 8 B ] 5 ; C ? 5 3 ^ 8 G = A 6 k F ] ^ Ó l d 4 A 4 ; = 7 5 3 3 g Y 8 B = > = 4 B [ ? X 5 A = 6 9 6 k d Y [ U l \ A 6 ; ? C 9 4 A ; 5 ; = 8 A d 4 A 4 ? 5 3 k \ d S ] l m 4 5 3 ; < ˆ Y 4 B = 7 5 3 d 4 A 4 ? 5 3 k Y d S ] l ] C ; ? 5 7 4 C ; = 7 5 3 6 k ] S f ^ l 2 3 5 A ; ^ 8 G = A 6 k 2 ` ] ^ l F 8 ? 4 A 6 = 7 6 d 4 A 4 ? 5 3 k _ d S ] l — = ; 5 9 = A 6 k — \ ^ Z l 1 n ! Ô $ n o p q r s t p q t q r p z v Õ ~ q w { v t p { p v r p | } ~ p  ‡ q q Ò x q € v q z r q Ö Z = ? ˆ Z = ? T 8 A ; 5 9 = A 5 A ; Z A 5 3 g 6 = 6 k Z \ _ l Z C ; 8 9 5 ; 4 B Y 4 ; < 8 B 6 k _ 8 D 8 ; = 7 6 l k Z f ^ [ l ƒ = 8 6 4 A 6 8 ? 6 k ƒ \ [ U l ƒ = 8 ; 4 ? ? 8 ? = 6 9 Z X 4 A ; _ 4 7 8 V 4 ? g 5 A B \ B 4 A ; = > = 7 5 ; = 8 A k ƒ \ [ ^ l T < 4 9 = 7 5 3 ^ < ? 4 5 ; Z X 4 A ; e 4 ; 4 7 ; = 8 A k T m ^ Z l T < ? 8 9 5 ; 8 X ? 5 : < g d T k T d T l ` T k T ` T l d T ˆ Y U k d T Y U l \ [ ] k \ [ ] l ` T ˆ Y U k ` T Y U l ` T ˆ Y U ˆ Y U k ` T Y U Y U l T 8 9 D C 6 ; = 8 A k T [ Y ƒ l S > > = 7 5 7 g ^ 4 6 ; = A X 8 > e = 6 = A > 4 7 ; 5 A ; 6 ˆ U 5 A = ; = Ñ 4 ? 6 k S F F \ T l S ` \ U Z Y = 7 ? 8 D = 8 3 8 X g k S ` \ U Z l S A X = A 4 4 ? = A X S × C = : 9 4 A ; k S ] d l F = 3 ; < k F \ ` l Y 5 A C > 5 7 ; C ? = A X S × C = : 9 4 A ; k Y Z ] f l Y 5 6 6 U : 4 7 ; 8 9 4 ; ? g k Y Z U U l  Á

` 5 D 8 ? 5 ; 8 ? g 2 5 ? ; = 7 = : 5 ; = 8 A k ` Z ƒ l Y 4 ; < 8 B 6 ˆ 2 C D 3 = 7 5 ; = 8 A _ 4 V = 4 • 4 ? k _ S — l T 8 9 9 C A = ; g [ > > = 7 4 ? k T [ Y [ l S G : 4 ? ; _ 4 V = 4 • 2 5 A 4 3 k S _ 2 l Y 4 ; < 8 B 6 T 8 9 9 = ; ; 4 4 Y 4 9 D 4 ? 5 A B ˆ 8 ? [ > > = 7 4 ? k Y T [ Y l [ ? X 5 A = Ñ 5 ; = 8 A 5 3 Z > > = 3 = 5 ; 4 k [ Z l _ 4 6 4 5 ? 7 < \ A 6 ; = ; C ; 4 S G : 4 ? ; _ 4 V = 4 • 4 ? k _ \ S _ l U 4 7 ; = 8 A [ > > = 7 4 ? k U S T [ l ^ 8 : = 7 Z B V = 6 8 ? k ^ Z l Z X ? = 7 C 3 ; C ? 5 3 F 4 ? ; = 3 = Ñ 4 ? 6 k Z F S _ l Z X ? = 7 C 3 ; C ? 5 3 Y = 7 ? 8 D = 8 3 8 X g k Z Y \ T l Z A ; = D = 8 ; = 7 6 k Z ] ^ \ l Z X ? = 7 C 3 ; C ? 5 3 U 8 = 3 k Z U [ \ l ƒ = 8 ; 4 ? ? 8 ? Z X 4 A ; 6 k ƒ \ [ ^ l T < 4 9 = 7 5 3 ^ 4 ? ? 8 ? Z X 4 A ; 6 k T m Y ^ l e ? C X T 8 6 9 4 ; = 7 6 k e T [ U l e = 6 = A > 4 7 ; 5 A ; 6 k e e \ U l e ? C X m C 9 5 A F 8 ? 9 C 3 5 ; = 8 A 6 5 A B Z B C 3 ; 4 ? 5 ; = 8 A 6 k e m f Y l S A V = ? 8 A 9 4 A ; 5 3 Y 4 ; 5 3 6  [ ; < 4 ? S 3 4 9 4 A ; 6 k S Y S ^ l S A V = ? 8 A 9 4 A ; 5 3 – 5 ; 4 ? k S – Z ^ l F 8 8 B ƒ 4 V 4 ? 5 X 4 6 k F ƒ S — l F 8 8 B Y = 7 ? 8 D = 8 3 8 X g k F Y \ T l F 8 8 B ] C ; ? = ; = 8 A k F ] f ^ l m 8 ? 9 8 A 4 6 k m [ _ Y l ` 5 D 8 ? 5 ; 8 ? g Z 7 7 ? 4 B = ; 5 ; = 8 A k ` Z T T l Y g 7 8 3 8 X g  F = 3 ; < k Y “ F ` l [ ; < 4 ? k [ ^ m _ l ‰ C 5 3 = ; g Z 6 6 C ? 5 A 7 4 ˆ T 8 A ; ? 8 3 k ‰ f Z ` l U ; 5 ; = 6 ; = 7 6 ˆ T < 4 9 8 9 4 ; ? = 7 6 ˆ ƒ = 8 9 4 ; ? = 7 6 k U ^ Z ^ l Y = 7 ? 8 D = 5 3 \ B 4 A ; = > = 7 5 ; = 8 A U g 6 ; 4 9 6 k Y \ U l Y = 7 ? 8 6 7 8 : g k Y \ T U l Y g 7 8 3 8 X g k Y “ T [ l [ ; < 4 ? k [ ^ m S _ l 2 T _ k 2 T _ l 2 4 ? 9 4 5 ; = 8 A k 2 S _ Y l _ 5 B = 8 A C 3 4 8 ; = B 4 6 k _ Z e \ [ l U : 4 7 = > = 7 \ 8 A S 3 4 7 ; ? 8 B 4 6 k U \ S l U : 4 7 ; ? 8 6 7 8 : g \ _ ˆ F ^ \ _ k U \ _ ˆ F ^ \ _ l ] \ _ k U ] \ _ l ] Y _ k U ] Y _ l Z Z k U 2 Z Z l f — ˆ — = 6 k U f — — l U : 4 7 ; ? 8 6 7 8 : g L \ T 2 k U 2 \ T 2 l – 4 ; T < 4 9 = 6 ; ? g

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Anli Gao Method Development Scientist, Biostatistician

Laboratory Services, University of Guelph

Direct line: 519-823-1268 x.57433

95 Stone Road West Guelph, N1H 8J7

Fax: 519-767-2640

E-mail: agao@uoguelph.ca

HIGHLIGHTS

 Multi disciplinary in microbiology, life history, population biology, molecular biology and biostatistics  Experience in method development for detection of pathogens in food  Experimental design, data analysis, and scientific presentation and publication  Team player and research project management

ACCOMPLISHMENTS

 Developed and validated methods for detection and isolation of pathogens in food matrices  Estimated measurement uncertainty for experiments with different designs  Provided consultation on experimental design, statistics, and data presentation to colleagues and graduate students  Authored/coauthored over 30 research papers on scientific journals, and over 10 conference presentations or/and abstracts  Supervised project students on research projects

EXPERTISE

 Experimental design and Data analysis

Experimental design and data collection for both parametric and nonparametric characteristics, spreadsheet skill, data analysis, statistics, multivariate analysis using SPSS and SAS, measurement estimation for various types of experiments, and result interpretation  Food Microbiology Method development for detection, isolation of bacterial strains from food, animal and environmental samples using variety of methods including centrifugation, selective culture, decontamination, immuno-capture separation, MALDI TOF system etc.  Molecular biology DNA extraction and purification, RFLP, PCR, Southern hybridization, method development for quick diagnosis of pathogenic organisms

WORK EXPERIENCE

University of Guelph, Laboratory Services Division, Guelph, Ontario Method Development Scientist, Biostatistician

2006 –

Research and development of methods for detection of foodborne pathogens. Method evaluation, verification and validation. Project management. Statistical support and consultation.

Research Associate

1998 – 2006

Laboratory Services is registered to the international quality standard ISO 9001:2000

Method development for sample preparation, isolation and speed detection of pathogens by culture and molecular techniques using immunomagnetic bead separation, centrifugations, etc. Investigation of the potential exposure of pathogen to human in milk using pasteurization, nested PCR, multicomplex PCR and specific culture methods. Experimental design, data analysis and statistics, report writing, and scientific publication. University of Guelph, Department of Pathobiology, Guelph, Ontario Research Assistant 1996 – 1998 Isolation and identification of microbe colonies in food and environmental samples. Plasmid preparation and identification. Investigate the differentiation of pathogenic and nonpathogenic E. coli strains using a variety of primer sets in PCR and hybridization. Ontario Ministry of Agriculture and Food Research Assistant 1996 Method development for analyzing the RFLP of E. coli DNA using pulse field gel electrophoresis. University of Guelph, Department of Zoology, Guelph, Ontario Fellow of National Sciences and Engineering Research Council of Canada 1993 – 1996 Analyzed and interpreted the geographical and genetic variation of harbor porpoise and the dispersal of populations. Morphometry of parametric and non-parametric characters using multivariate analysis. Age calibration, regression analysis, growth and reproduction, field survey and strategy study for conservation of marine mammals in Bay of Fundy. 1984 – 1993 Established a molecular laboratory. RFLP of mitochondrial DNA in geographical populations of mammals. Marine mammal age determination, life history, morphology and adaptation to habitats. Conservation of endangered species in Yangtze River. Taught Zoology, Cetacean Biology and various subjects including “Important Computer Software for Graduate Students of Science” and “Introduction of Multivariate Analysis” to graduates. Supervised the field surveys and lab work in several scientific research programs on marine mammals. Nanjing Normal University, Department of Biology, Nanjing, P.R. China Lecturer

EDUCATION

B.Com., Department of Computing and Information Science, University of Guelph, 2002 Ph.D., Vertebrate Biology, Department of Biology, Nanjing Normal University, 1991

M.Sc., Department of Biology, Nanjing Normal University, 1984 B.Sc., Department of Biology, Shaanxi Teacher's University, 1982

Laboratory Services is registered to the international quality standard ISO 9001:2000

Yang, G., K. Zhou, A. Gao and Q. Chang. 1998. A study on the life table and dynamics of three finless porpoise populations in the Chinese waters. Acta Theriologica Sinica 18(1):1-7.

Gao, A. and D.E. Gaskin. 1998. Geographical variation in harbour porpoise, Phocoena phocoena , evaluated by discriminant analysis. Marine Mammal Science 14(3):649-652.

Gyles, C., R. Johnson, A. Gao, K. Ziebell, D Pierard, S. Aleksic and P. Boerlin. 1998. Association of enterohemorrhagic Escherichia coli hemolysin with serotypes of Shiga-Like-Toxin-Producing Escherichia coli of human and bovine origins. Applied and Environmental Microbiology 64(11):4134- 4141.

Zhou, K., J. Sun, A. Gao and B. Würsig. 1998. Baiji (Lipotes vexillifer) in the lower Yangtze River: movements, numbers threats and conservation needs. Aquatic Mammals 24(2):123-132.

Gao, A. and D.E. Gaskin. 1996. Geographical variation in metric skull characters among proposed subpopulations and stocks of harbour porpoise, Phocoena phocoena , in the western North Atlantic. Marine Mammal Science 12(4):516-527.

Gao, A. and D.E. Gaskin. 1996. Nonparametric morphometry of the skull of harbour porpoise, Phocoena phocoena , in western North Atlantic and eastern Pacific. Canadian Journal of Zoology 74:2199-2205.

Gao, A., K. Zhou and Y. Wang. 1995. Geographical variation in morphology of bottlenose dolphins ( Tursiops ) in Chinese waters. Aquatic Mammal 21(2):121-135.

Gao, A. and K. Zhou. 1995. Geographical variation of external measurements and three subspecies of Neophocaena phocaenoides in Chinese waters. Acta Theriologica Sinica 15(2):81-92.

Gao, A. and K. Zhou. 1995. Geographical variations of skull among the populations of Neophocaena in Chinese waters. Acta Theriologica Sinica 15(3):161-169.

Gao, A. and K. Zhou. 1995. Geographical variations of postcranial skeleton among the populations of Neophocaena in Chinese waters. Acta Theriologica Sinica 15(4):246-253.

Zhou, K. and A. Gao. 1995. Semi-natural reserve is the best possible approach to rescuing the baiji. IBI Report 5:57-61.

Zhou, K. and A. Gao. 1994. General Biology of the baiji, Lipotes vexillifer . In: Zhou, K., Sue Ellis, S. Leatherwood, M. Bruford and U. Seal (eds.), Baiji Population and Habitat Viability Assessment. pp. 103- 119. Zhou, K., Y. Hou, A. Gao, T. Kamiya and R. Tatrukawa. 1994. Heavy metals in tissues of finless porpoise in the East China Sea. Sixtieth Anniversary of the founding of China Zoological Society. Memorial volume dedicated to the Hundredth anniversary of the Birthday of the Late Prof. Sisan Chen (Z. Chen) , pp. 201-211. Bruford, Mike, D. Wang, A. Gao, Q. Liu, and Y. Wang. 1994. Population biology and modelling. In: Zhou, K., Sue Ellis, S. Leatherwood, M. Bruford and U. Seal (eds.), Baiji Population and Habitat Viability Assessment. pp. 121-142.

Gao, A. and K. Zhou. 1993. Notes on the classical literatures and contemporary researches on the finless porpoise ( Neophocaena phocaenoides ). Acta Theriologica Sinica 13(3):223-234.

Laboratory Services is registered to the international quality standard ISO 9001:2000

Gao, A. and K. Zhou. 1993. Growth and reproduction of three populations of finless porpoise, Neophocaena phocaenoides , in Chinese waters. Aquatic Mammals 19(1):3-12. Zhou, K., A. Gao and J. Sun. 1993. Notes on the biology of the finless porpoise in Chinese waters. IBI Reports (4):69-74.

Sun, J., K. Zhou and A. Gao. 1993. A report on birds sighted along the lower reaches of the Yangtze River. Chinese J. Zool. 29(1):23-28.

Gao, A. and K. Zhou. 1992. Sexual Dimorphism in the baiji, Lipotes vexillifer . Can. J. Zool. 70:1484- 1493.

Zhou, K., J. Sun, Y. Hua and A. Gao. 1992. Population monitoring and photo-identification study of the baiji, Lipotes vexillifer , in the lower Yangtze. Proceedings of 23rd Annual IAAAM conference, May 18- 22. Hong Kong. Gao, A., J. Sun and K. Zhou. 1988. The possibility of using photographic techniques to identify individual baiji, Lipotes vexillifer . Working paper SC/A88/P6, Symposium and Workshop on the Use of Non-Lethal Techniques, Especially Photo-Identification Techniques, to Estimate Cetacean population parameters. 28 April - 4 May, 1988, La Jolla, California.

Zhou, K. and A. Gao. 1988. Individual and mass stranding of sperm whale, Physeter macrocephalus , in China (abstract, in English). Asia-Pacific theriological symposium, 1988, Beijing.

Gao, A. and K. Zhou. 1987. On the retinal ganglion cells of Neophocaena and Lipotes . Acta Zool. Sinica , 33(4):316-322.

Gao, A. and K. Zhou. 1986. Anatomical and histological studies of the eyes of the finless porpoise, Neophocaena phocaenoides . Acta Zool. Sinica , 32(3):248-254.

Wang, Q., D. Wang, J. Liu, and A. Gao. 1984. The rodents on farm land in the east part of Shaanxi in autumn. Chinese J. Zool. (6):33-36.

Laboratory Services is registered to the international quality standard ISO 9001:2000

PUBLICATIONS and SELECTED PRESENTATIONS

Gao, A., P. Martos, S. Balamurugan. Estimation of Measurement Uncertainty of Bacterial Enumeration in Experiments with Factorial Design (Poster P-T-056 and oral presentation). AOAC 128 th Annual Meeting and Exposition, Boca Raton Resort and Club, Boca Raton, FL. Sep. 7-10, 2014. Gao, A., J. Fischer-Jenssen, S. Lee, J. Li, C. Leon-Velarde, S. Chen, Perry Martos. Identification of Foodborne Organisms Using MALDI TOF MS and 16S rRNA Gene Sequence-based Method (Poster P- T-077). AOAC 128 th Annual Meeting and Exposition, Boca Raton Resort and Club, Boca Raton, FL. Sep. 7-10, 2014. Saleh-Lakha, S., V. G. Allen, J. Li, F. Pagotto, J. Odumeru, E. Taboada, M. Lombos, K. C. Tabing, B. Blais, D. Ogunremi, G. Downing, S. Lee, A. Gao, C. Nadon, S. Chen. 2013. Subtyping of a Large Collection of Historical Listeria monocytogenes Strains from Ontario, Canada, by an Improved Multilocus Variable-Number Tandem-Repeat Analysis (MLVA). Applied and Environmental Microbiology 79:6472–6480. Chibeu, A., Louise Agiusc, Anli Gao, Parviz M. Sabour, Andrew M. Kropinski, S. Balamurugan. 2013. Efficacy of bacteriophage LISTEX™P100 combined with chemical antimicrobials in reducing Listeria monocytogenes in cooked turkey and roast beef. International Journal of Food Microbiology 167:208– 214. Ramsahoi, L., A. Gao, M. Fabri, and J. A. Odumeru. 2011. Assessment of the application of an automated electronic milk analyzer for the enumeration of total bacteria in raw goat milk. Journal of Dairy Science 94 :3279–3287. Gao, A., J. Odumeru, M. Raymond, S. Hendrick, T. Duffield, L. Mutharia. 2009. Comparison of milk culture, direct and nested polymerase chain reaction (PCR) with fecal culture based on samples from dairy herds infected with Mycobacterium avium subsp. paratuberculosis. The Canadian Journal of Veterinary Research 73:58–64. Gao, A., L. Mutharia, M. Raymond, and J. Odumeru. 2007. Improved template DNA preparation procedure for detection of Mycobacterium avium subsp. paratuberculosis in milk by PCR. Journal of Micribiological Methods 69:417-420. Gao, A., J. Odumeru, M. Raymond, and L. Mutharia. 2005. Development of improved method for isolation of Mycobacterium avium subsp. paratuberculosis from bulk tank milk: Effect of age of milk, centrifugation and decontamination. Canadian Journal of Veterinary Research 69:81-87.

Gao, A., L. Mutharia, S. Chen, K. Rahn, and J. Odumeru. 2002. Effect of pasteurisation on survival of Mycobacterium paratuberculosis in milk. Journal of Dairy Science 85:3198-3205.

Odumeru, J., A. Gao, M. Raymond, and L. Mutharia. 2001. Use of the bead beater for preparation of Mycobacterium paratuberculosis template DNA. Canadian Journal of Veterinary Research 65:201-205.

Todd, E., R. Szabo, J. MacKenzie, A. Martin, K. Rahn, C. Gyles, A. Gao, D. Alves, and A. Yee. 1999. Application of a DNA hybridization-hydrophobic-grid membrane filter method for detection and isolation of verotoxigenic Escherichis coli. Applied and Environmental Microbiology 65:4775-4780.

Laboratory Services is registered to the international quality standard ISO 9001:2000

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