August 30, 2016 SPADA Meeting Book

Background: Poxvirus 101

 Family of large, double stranded DNA viruses  Within genera, antigenic similarity: t ti cross pro ec on  Complex viruses, cytoplasmic lifecycle  Genus Orthopoxvirus:  90-98% nucleotide identity across species V i l V i i C  ar o a, acc n a, owpox, Monkeypox – all can cause human disease • Variola evolved to be a sole human pathogen: SMALLPOX

 Camelpox, Ectromelia, Taterapox are NOT known to cause human disease

Objectives

• Smallpox introduction

• Development of smallpox clinical diagnostics

• Developing a framework for considering environmental smallpox detection/testing

– Results of 2016 Standard Method Performance Requirements for Detection of Variola virus DNA in collection buffers from aerosol collection devices for DoD applications