August 30, 2016 SPADA Meeting Book

Virus Characterization

“Genomic sequencing and limited study of Variola virus surface proteins derived from geographically dispersed specimens is an essential foundation for important future work. Such research could be carried out now, and could require a delay in the destruction of known stocks, but would not necessitate their indefinite retention.*”

* Assessment of Future Scientific Needs for Live Variola Virus ; N.A.Press (1999)

Maximum Likelihood Analysis of Single Nucleotide Matrices ( Variola virus )

48 isolates passaged 2-3 times to high titer master d

African minor AND African major

NON- WEST AFRICA

see stocks

DNA extracted Sequenced using primer walking methodology

BANGLADESH NEPAL INDIA SUMATRA MIDDLE EAST

Asian Variola major

EAST ASIA ORIENT W AFRICA BRAZIL

Alastrim minor

Li Y. et.al. PNAS 2007;104:15787-15792 Esposito et. al Science 2006