August 30, 2016 SPADA Meeting Book

Post-event

• Need to assess what needs for testing will be: – One scenario: greatest needs in beginning, and near end of smallpox re-eradication • May need to test more “low suspicion samples” Sensitivity vs specificity – .

Issues Relevant to Implementation of Smallpox Diagnostics

• Biosafety:

– containment; – Standard vs. Universal vs. Airborne precautions; – Vaccination • Biosecurity • Reagents – WHO, CDC, select agent recommendations on use of Variola virus DNA • Centralized/regionalized testing – Transportation of specimens • QA/QC, Proficiency testing: implementation • Communication between clinician/epidemiologists/laboratory – Clinical history, case patient photos • Development of a disease confirmatory algorithm – Screening tests, confirmatory tests: regional vs. centralized – Presumed positive, Confirmed Positive – Communication of results, and public health response – Role of viral isolation by culture