August 30, 2016 SPADA Meeting Book

Inclusivity Panel

Complementary Set: Exclusivity Panel - near neighbor ( Orthopoxviruses ) Species Strain Ectromelia virus Moscow Monkeypox virus 79-0266 Monkeypox virus 79-0005 Camelpoxvirus LLC Camelpoxvirus V78-I-903

Species

Strain

Sample Crude Crude Crude Crude Crude Crude Crude Crude

102 103

Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus

66-39 7124 7125 72-119 73-175 77-1605 Bombay Congo Eth-17 Harper Harvey Higgins Hinton

Crust Crude Crude Crude Crude Crude Crude Crude Crude Crude Pure Crude Crude Crude Crude Crude Pure Crude Crust Crude Crude Crude Crude Crude Crude Crude Crude Crude Crude

Brazil-Garcia

Brighton

Cowpoxvirus Taterapoxvirus Vaccinia virus Vaccinia virus Vaccinia virus Vaccinia virus Vaccinia virus Skunkpovirus

(Gerbilpox)

Lister VTH Wyeth

Heidelberg

WYH pGS62-9-v1-1-1

Horn Horn

Rabbitpoxvirus

K1629

Kali Mathu Kembula

Exclusivity Panel - other rash-causing illnesses Species Strain V i ll Z t Vi W b t ar ce a os er rus e s er

Minnesota 124

MS Lee Nepal

HFEM conorii

Herpes Simplex Virus-1

New Dehli

Nigeria Kuclano

Rickettsia

Nur Islam Rumbec Shahzamon Solaiman

Exclusivity Panel - other negatives Species Strain Myxoma

Stillwell V68-59 V70-222 V70-228

Human tissue culture cells

Sup-T

Variolator-4

Monkey kidney tissue culture cells

BSC-40

Yamada

 Validated diagnostic real- time PCR assays Variola virus : Diagnostic Creation and Validation  Target multiple regions of the genome  Target sequences specific to Variola virus  Cowpox virus sequences acquired after validation  Exhibit extensive phylogenetic diversity  Contain certain regions

previously thought to be specific to Variola virus • Now assays predicted to

cross-react with Cowpox virus

D. Carroll, et. al PLoS One. 2011;6(8):e23086