Candidates for 2016 ERP of the Year

ERP PROFILE SUMMARIES

Primary and Secondary Evaluation of Method SJW-05

SJW-05:

Simultaneous Determination of Protopseudohypericin, Pseudohypericin, Protohypericin, and HypericinWithout Light Exposure

Steven F. Baugh, Industrial Laboratories, 4045 Youngfield St, Wheat Ridge, CO 80033

Author(s):

S UMMARY OF M ETHOD : G ENERAL C OMMENTS :

This manuscript does not describe a method of analysis for determination of analytes in St. John’s wort per se, but rather examines the feasibility of employing a mathematical conversion for accurate determination of the naphthodianthrones without light exposure by employing a simplified protocol in combination with using a conversion factor.

P ROS /S TRENGTHS : This method could be a way to simplify sample preparation.

C ONS /W EAKNESSES : The paper does not describe a method of analysis. Data supporting use of a mathematical conversion with a simplified extraction protocol is presented. The focus is naphthodianthrones and does not apply to any other stated compounds of interest in St. John's wort.The method does not meet the requirements stated in the SMPR.

EXPERT REVIEW PANEL VOTE AND RECOMMENDATION

MOTION: Not to consider this method for First Action Official Method status. Brown, Reif(Unanimous) Motion Passed

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