Candidates for 2016 ERP of the Year

ERP PROFILE SUMMARIES

Primary and Secondary Evaluation of Method SJW-11

SJW-11:

Determination of hyperforin in St. John’s wort Extract and Plant Material by High- performance Liquid Chromatography (INA Method 112.001)

Institute for Nutraceutical Advancement Methods Validation Program

Author(s):

S UMMARY OF M ETHOD : G ENERAL C OMMENTS : This is a HPLC/UV method used to test the hyperforin content in St. John’s wort. The sample preparation involves sonication and centrifugation prior to analysis. The method is a fairly typical HPLC method; however, it does not include any validation data. The majority of well equipped analytical labs could run this assay. P ROS /S TRENGTHS : This is a simple and straightforward HPLC method that most analytical labs would have the analytical equipment needed to run this method.

C ONS /W EAKNESSES : The Hyperforin in the solution is not stable. This method does not include any validation data.

EXPERT REVIEW PANEL VOTE AND RECOMMENDATION

MOTION:Not to consider this method for First Action Official Method status. Reif, Schaneberg (Unanimous) Motion Passed

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