Carn-06_Book_Final

Carn-06 - Validation Report FOR ERP USE ONLY DO NOT DISTRIBUTE

4 ≤ 8 ,2 Therefor no difference between the results has been demonstrated on a 95% confidence level. To compare our results with data on 1849A and 1845A published at the 2 nd. Vita- min conference in Copenhagen 2 the data has been evaluated with at t-test (un- equal sample size, equal variance).

where

Using the zero hypotheses that no difference between the two data sets is ob- served. Sample: NIST SRM 1849A Matrix: Infant/adult nutritional formula. Species Result n Reference value n Comment carnitine 158 ± 4 mg/kg 8 149 ± 1 mg/kg 20 Total carnitine

At 26 degrees of freedom the t value of 2,0555 is critical for α=0,05

9 ≥ 2 ,1 The Zero hypotheses are rejected on a 95% confidence level. There is a signifi- cant difference between the results. The explanation is that alkaline hydrolysis is more efficient in hydrolysing the car-

2 Data presented at the 2 nd. International vitamin conference in Copenhagen 2012.