Carn-06_Book_Final

Carn-06 (Choline Validation Report) FOR ERP USE ONLY DO NOT DISTRIBUTE

Abbreviations and synonyms:

Abbreviations: NMKL.................Nordisk Metodikk Komité for Næringsmidler ISO ....................International Organization for Standardization SOP...................Standard Operating Procedure ANAVA ..............Analysis of Variance LOD ...................Limit of Detection LOQ...................Limit of Quantitation n ........................Number of replicates LC......................Liquid Chromatography MS .....................Mass spectroscopy R 2 .......................Squared regression coefficient RSD%................Relative Standard Deviation in Percent CI .......................Confidence intervals S r .......................Repeatability (standard deviation within days) S R ......................Reproducibility (standard deviation between days) SRM ..................Certified reference material NIST ..................National Institute of Standards and Technology Synonyms: t-test .................. Student’s t -test This validation report is conducted in accordance to our internal instruction 211.01 “Validering af analysemetoder i Vitamincenter (in English - Validation of analytical methods in the Vitamin Centre” This instruction defines the experimental design used in the current validation, which is based on NMKL Procedure No. 4: Validation of chemical analytical methods (2009, 3 rd edition). The Eurofins Steins Laboratory in Vejen is accredited according to ISO 17025. Quality statement:

Introduction:

This method was developed in the vitamin centre. The extraction is based on an old internal Eurofins method for determination of choline.

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