Carn-06_Book_Final

Carn-06 (Choline Validation Report) FOR ERP USE ONLY DO NOT DISTRIBUTE

Appendix 1: Linearity

Choline:

Actual Concentration (ng/g)

IS Actual Concentration (ng/g)

Area (counts)

IS Area (counts)

Concentration ratio

Area ratio

std 1 std 2 std 3 std 4 std 5 std 6 std 7 std 8 std 9 std 1 std 2 std 3 std 4 std 5 std 6 std 7 std 8 std 9

4,568

226,0465 233,9615

3500,1 7158,5

95414,3 0,020208231 0,036682647 100386,5 0,038466585 0,071309878 93116,9 0,173867577 0,287132919 92623,6 0,318615155 0,521775092 99629,3 0,624685581 0,968733239 90238,0 1,118062969 1,820782019 82948,7 2,361487047 3,785176869 76108,7 4,316266516 7,069375187 71076,6 6,666763474 10,86967718 93352,2 0,020208231 0,040898639 96396,4 0,038466585 0,064023053 94439,2 0,173867577 0,277703701 93023,6 0,318615155 0,517376265 92762,0 0,624685581 0,997698961 91383,4 1,118062969 1,804405624 85948,8 2,361487047 3,713790633 76994,4 4,316266516 6,907796781 71884,0 6,666763474 10,6361857

8,9997 37,8086 73,3656 144,8371 253,9907 545,4022 990,3721 1535,7123 8,9997 37,8086 73,3656 144,8371 253,9907 545,4022 990,3721 1535,7123 4,568

2,17E+02 26736,9 230,264 48328,7 231,856 96514,2 227,1703 164303,6 230,9571 313975,4 229,4511 538040,9 230,3535 772579,6 217,4563 26226,1 230,264 48128,2 231,856 92548,6 227,1703 164892,7 230,9571 319196,0 229,4511 531861,6 230,3535 764571,2 226,0465 233,9615 3818,0 6171,6

SUMMARY OUTPUT

Regression Statistics

Multiple R R Square

0,999881525

0,999763065 Adjusted R Square 0,999748257 Standard Error 0,057753575 Observations 18

ANAVA

df Significance F Regression 1 225,1882143 225,1882143 67513,0793 1,95064E-30 Residual 16 0,053367606 0,003335475 Total 17 225,2415819 SS MS F

Coefficients

Std. Error

t Stat

P-value

Lower 95%

Upper 95%

Intercept

-0,004021295 0,017370086 -0,23150690 0,819855739 -0,04084423 0,032801643

Concentration ratio 1,613426982 0,006209482

259,83279 1,95064E-30 1,60026346 1,626590496

RESIDUAL OUTPUT Observation Predicted Area

Residuals

1 2 3 4 5 6

0,028583211 0,008099436 0,058041732 0,013268147 0,276501345 0,010631574 0,510040993 0,011734099 1,003863276 -0,035130037 1,799891666 0,020890352