Chemistry-PTM-OMA_Modules_1-4

Examples of Robustness Parameters Parameters that should be varied will be method dependent.    Examples of parameters that might be varied are as follows:

• temperature at any temperature  critical step  (e.g., storage, extraction,  incubation, mixing) • times for any time critical step (e.g.,  storage, extraction, incubation,  mixing) • order of reagent addition

• test portion mass/volume • laboratory sample holding time  • test portion/solvent ratio • reagent volume(s) • reagent concentration