Chemistry-PTM-OMA_Modules_1-4

Method Parameter Robustness Examples The greater the reduction in combinations from a full factorial, the wider the variation in method parameters  is needed to examine the effect of the parameter variation.

Robustness Test  Parameter

Baseline Value  (Package Insert  Instructions)

Low Value

High Value

Full factorial

4.5 g 4.0 g

5.5 g 5.0 g

Test portion mass

5 g ± 0.5g

Plackett‐Burnam

Full factorial

110 min

130 min

Extraction time

120 min ± 10 min

Plackett‐Burnam

100 min

140 min

Full factorial

10 min

20 min

Time between  extraction and  instrument reading

15 min ± 5 min

Plackett‐Burnam

7 min

25 min