2019 Kombucha ERP - Review Book

Does the method perform according to the method"s applicability as written? If No, please explain why the method did not perform according to the method"s applicability as written.

Yes

2. SAFETY CONCERNS

Are there any safety concerns identified while using or regarding use of the method? If Yes, please identify the safety concerns regarding the use of this method.

Yes. Some of the chemicals used in the method are flammable, the safe handling of these and any other hazardous chemicals may be pointed out in the method.

3. STATISTICAL REVIEW

Is the documentation for the statistical review prior to AOAC First Action Official Methods status available and completed?

Yes

4. REFERENCE MATERIALS

5. SINGLE LABORATORY VALIDATION

Is there data demonstrating linearity, accuracy, repeatability, LOD/LOQ? If No, please explain.

Yes

6. REPRODUCIBILITY/UNCERTAINTY AND PROBABILITY OF DETECTION

Does the available documentation demonstrate Reproducibility/Uncertainty and Probability of Detection? If yes, please specify the information provided to support the reproducibility of this method as written.

1. Not apparent using conventional norms used by many national and international standard guidelines. 2. The MLT study could provide only four valid data from participating laboratories for studies samples. 3. Two of the six collaboratively studied samples provided RSDR values considrably higher than the SMPR requirements. 4. Thus only four laboratory could provide collaborative data for four samples meeting SMPR requirements with respect to the method reproducibility. 5. Was blind coding of samples for collaborative study done per AOAC protocol - not clear fronm the avalable information.

7. FINAL ACTION REQUIREMENTS