ERP Micro December 2019

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Table 2019.2A (cont’d.) . Comparative results for the Detection of Listeria monocytogenes in full-cream goat milk cottage cheese (8.4% fat) test portions 1 by the GENE-UP ® Listeria monocytogenes 2 (LMO 2) method vs. ISO 11290-1/Amd.1 in a collaborative study (high inoculum level) 2

Candidate presumptive (CP)

C vs. R CP vs. CC

Candidate confirmed (CC)

Candidate result (C)

Reference method (R)

Statistic

Matrix

Laboratory

POD (CP) 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 N/A 1.00 1.00 1.00 1.00 1.00 1.00 0.97 1.00 0.00 0.00 0.17 0.00 0.00 0.17 0.00 0.00 0.24

POD (CC) 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 N/A 1.00 1.00 1.00 1.00 1.00 1.00 0.97 1.00 0.00 0.00 0.17 0.00 0.00 0.17 0.00 0.00 0.24

POD (C) 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 N/A 1.00 1.00 1.00 1.00 1.00 1.00 0.97 1.00 0.00 0.00 0.17 0.00 0.00 0.17 0.00 0.00 0.24

POD (R) 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 N/A 1.00 1.00 1.00 1.00 1.00 1.00 0.97 1.00 0.00 0.00 0.17 0.00 0.00 0.17 0.00 0.00 0.24

dLPOD (C,R)

dLPOD (CP,CC)

1 2 3 4 5

8 8 8 8 8 8 8 8 8 8 8 8 8 8 8

0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 N/A 0.00 0.00 0.00 0.00 0.00 0.00 -0.03 0.03

Full- Cream

Goat Milk

Cottage Cheese (8.4% Fat)

6 a 7 a 8 a

CFU/ Test Portion

N/A

11 a

12 13 14 15 16

9.2 7.2 11.4

Estimate

All

120

LCL UCL LCL UCL LCL UCL UCL LCL s r b s L c s R d

P T e 1.0000 a Collaborator Data Not Included in Statistical Analysis, b Repeatability Standard Deviation, c Among-Laboratory Standard Deviation, d Reproducibility Standard Deviation, e P T 1.0000 1.0000 1.0000

3 4

Value = Homogeneity test of laboratory PODs

N - Number of Test Portions; X – Number of Positive Test Portions; LCL – Lower Confidence Limit; UCL – Upper Confidence Limit

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