ERP Micro December 2019

Inoculation Level per Organism 0 cfu/375g

Matrix

Inoculation Organisms

Replicates

Test Portion

Method

5

375g 375g 375g

Raw beef trim

E. coli O157:NM (ATCC 1 700376)

0.2–2 cfu/375g

GDS EHEC

20

2–10 cfu/375g

5 5

0 cfu /25g

25g 25g 25g

Raw beef trim

E. coli O157:NM (ATCC 700376)

MLG 5C.00

0.2–2 cfu/25g 2–10 cfu/25g

20

5 5

0 cfu/375g

375g 375g 375g

Frozen finely textured beef Frozen finely textured beef

E. coli O157:H7 (ATCC 43888)

0.2–2 cfu/375g

GDS EHEC

20

2–10 cfu/375g

5 5

0 cfu /25g

25g 25g 25g

E. coli O157:H7 (ATCC 43888)

MLG 5C.00

0.2–2 cfu/25g 2–10 cfu/25g

20

5 5

0 cfu /375g

375g 375g 375g

Raw green onions

E. coli O157:H7 (ATCC 35150)

0.2–2 cfu/375g

GDS EHEC

20

2–10 cfu/375g

5 5

0 cfu /25g

25g 25g 25g

Raw green onions

E. coli O157:H7 (ATCC 35150)

FDA BAM

0.2–2 cfu/25g 2–10 cfu/25g

20

5

Surface Area (FSIS)

Inoculation Organisms

Inoculation Level

Type

Replicates

Sampling Device

Method

0 cfu Low High

5

E. coli O157:H7 (ATCC 43890)

MicroTally TM Cloth

Carcass cloth

280 sq. inch

GDS EHEC

20

5

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