ERP Micro December 2019

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3.1.1 For inclusivity, select at least 50 isolates of E. coli strains O157:H7 and O157:NM

Formatted: Outline numbered + Level: 3 + Numbering Style: 1, 2, 3, … + Start at: 1 + Alignment: Left + Aligned at: 1" + Indent at: 1.5"

( Table 1 ).

3.1.2 Culture each strain in BioControl mEHEC® media for 10–18 h at 41–43°C. Dilute each isolate, if necessary, in sterile enrichment media to 100 times the LOD 50 of

the GDS O157 assay.

3.1.3 Blind-code and randomize inclusivity isolates so that the analyst does not know

the identity of the test samples.

3.1.4 Test one replicate of each isolate with the GDS O157 assay.

3.1.5 Decode and tabulate results by strain.

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3.1.6 Report inclusivity data as the number of strains detected out of the total number tested. For example, “Of the 50 inclusivity strains tested, 48 were correctly identified and 2 were not correctly identified. Those strains not correctly

identified were…”

3.1.7 Report the source and origin of all strains tested.

Table 1. Inclusivity Strains

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