ERP Micro December 2019

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3.5.5 Prepare a summary table of POD values, dPOD values, and confidence intervals

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by matrix and concentration.

3.5.6 Plot POD C

, POD R

, and dPOD C

with 95% confidence intervals by concentration for

each matrix.

4.04 Report

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The validation study report is to be submitted by the method developer at the completion of data collection. This report is to incorporate both the results generated through internal testing at the method developer laboratory. The assigned expert reviewers and the general referee will review this study report. Once reviewed and any revisions are made, the expert review panel (ERP) will determine if the modification is acceptable for Official Methods of Analysis status. If acceptable, the method developer should submit the report for publication in the Journal of the AOAC

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INTERNATIONAL .

5.05 References

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1. AOAC INTERNATIONAL Methods Committee Guidelines for Validation of Microbiological Methods for Food and Environmental Surfaces (2012) Official Methods of Analysis of AOAC INTERNATIONAL , 19th Ed., Appendix J, AOAC INTERNATIONAL, Gaithersburg, MD, 2. United States Department of Agriculture-Food Safety and Inspection Service Microbiology Laboratory Guidebook , 5 .09C.00 , Detection, Isolation and Identification of Top Seven Shiga Toxin-Producing Escherichia coli (STECs) from Meat Products and Carcass and Environmental Sponges Detection, Isolation and Identification of O157:H7 from Meat Products and Carcass and Environmental Sponges ( 20152019 ) https://www.fsis.usda.gov/wps/wcm/connect/7ffc02b5- http://www.eoma.aoac.org

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3d33-4a79-b50c-81f208893204/mlg-5.pdf?MOD=AJPERES

https://www.fsis.usda.gov/wps/wcm/connect/51507fdb-dded-47f7-862d-ad80c3ee1738/MLG-

5.pdf?MOD=AJPERES

3. United States Food & Drug Administration Bacteriological Analytical Manual , Chapter 4A,

Diarrheagenic Escherichia coli (2018)

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https://www.fda.gov/food/foodscienceresearch/laboratorymethods/ucm070080.htm

2.

4. Least Cost Formulations MPN Calculator

http:// www.lcfltd.com/customer/LCFMPNCalculator.exe

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3.5. United States Department of Agriculture-Food Safety and Inspection Service, Sampling Verification Activities for Shiga Toxin-Producing Escherichia coli (STEC) in Raw Beef Products (2015) https://www.fsis.usda.gov/wps/wcm/connect/c100dd64-e2e7-408a-8b27-

ebb378959071/10010.1.pdf?MOD=AJPERES 39 5. Least Cost Formulations, Ltd., AOAC Binary Data Interlaboratory Workbook 40 http://lcfltd.com/aoac/aoac-binary-v2-2.xls 41

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