ERP Micro December 2019

surfaces. Method: The GENE-UP ® method was evaluated in a multi-laboratory study as part of the AFNOR NF VALIDATION certification process using unpaired test portions for one food matrix, full-cream goat milk cottage cheese (8.4% fat). The candidate method was compared to the ISO 11290-1/Amd.1 reference method. Sixteen participants from 15 laboratories throughout the European Union participated. Three levels of contamination were evaluated: a non- inoculated control level (0 CFU/test portion) , a low contamination level (~2 CFU/test portion) and a high contamination level (~10 CFU/test portion). Data from that study were analyzed according to the Probability of Detection (POD) statistical model as presented in the AOAC validation guidelines. The difference in laboratory POD (dLPOD C ) values with 95% confidence interval across collaborators was calculated for each level between the candidate and reference method results, and between the candidate presumptive and confirmed results. Results : The dLPOD C values with 95% confidence interval between the candidate and reference method results were; -0.02 (-0.07, 0.03), -0.08 (-0.31, 0.16) and 0.00 (-0.03, 0.03) for the non-inoculated, low and high contamination levels respectively. Conclusion : The dLPOD C results demonstrate no difference in performance between the candidate method and reference method for the matrix evaluated. _________________________________________________________________________ Listeria , a Gram-positive bacterium, that is ubiquitous in soil, water, and several animals intended for human consumption, can be found in food processing plants from a variety of food products including dairy, meat, vegetables, and seafood (1). Two species of Listeria , L. monocytogenes and L. ivanovii, are considered human pathogens and have been associated with illness, although L. monocytogenes is known as the primary causative agent of illness (2).

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