ERP Micro December 2019

AOAC Official Method 2019.xxx Listeria species in a Variety of Foods and Select Environmental Samples: GENE-UP ® Listeria spp. 2 (LIS 2) Test Method First Action 2019

Applicable to detection of Listeria spp ( monocytogenes, innocua, ivanovii, seeligeri, and welshimeri ) in 25 g test portions of deli ham, deli turkey, beef hot dogs, breaded chicken nuggets, fresh bagged spinach, fresh bagged lettuce, cooked shrimp, smoked salmon, vanilla ice cream, Mexican soft cheese, pork rillettes, raw milk, salmon offcuts, mixed precooked vegetables, mixed salad and process water; 100 g test portions of whole liquid egg; 125 g test portions of deli ham & smoked salmon; 375 g test portions of whey powder; stainless steel surface sponges

See Table 2019.1A for a summary of results of the inter-laboratory study. See Table 2019.2A for detailed results of the inter-laboratory study

A. Principle

The GENE-UP ® Listeria spp. 2 (LIS 2) kit is designed for use with the GENE-UP ® Thermocycler. The GENE-UP ® LIS 2 kit contains all of the necessary components for PCR, including sample-specific primers and probes and an internal amplification control. The GENE- UP ® Thermocycler detects fluorescence at several wavelengths (channels) to allow for multi- target detection in the same reaction vessel. The fluorescent signal from a sample is recorded in channel 640, while the fluorescent signal for an internal amplification control is recorded in channel 705. The software automatically interprets the results for the internal amplification

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