ERP Micro December 2019

Note: Visually check the tips to confirm the absence of beads, bubbles, and for correct volume of lysate. Note: For negative control procedure, use 10 μL of control buffer instead of lysed sample. (d) Place and seal the strip caps onto each strip tube using the GENE‑UP ® Lysis Tube Remover Tool. If less than eight caps are required, the caps can be cut apart, and only the used caps are placed onto the strip tubes. (e) Place the GENE‑UP ® PCR Tube Holder containing the PCR tubes in the plate centrifuge. (f) Balance the centrifuge. (g) Spin for 10 s. (h) The plate is now ready to be processed in the GENE‑UP ® instrument and is stable for 2 h at 15–25°C. Note: The lysis tubes can be removed from the GENE‑UP ® Lysis Tube Holder using the GENE‑UP ® Lysis Tube Remover Tool. The GENE‑UP ® Lysis Tube Holder is reusable, but the used lysis tubes should be disposed of accordingly. (i) Please refer to the appropriate GENE‑UP ® instrument user manual for instructions to start a run, view results, and use the GENE‑UP ® Routine software.

G. Results and Interpretation

(a) Results are automatically interpreted once the PCR run is completed. The routine software interprets data for each sample and gives a positive, negative, or inhibited result as indicated in the following table

Listeria spp.

Internal amplification

Result

16