ERP Micro December 2019

Comments on the Safety of the Method:

The following safety instructions and precautions are included in the OMA section of the manuscript:

C. General Instructions

(a) The GENE-UP® Listeria spp. 2 (LIS 2) kit is for professional use only with the GENE UP® Lysis kit. (b) Comply with Good Laboratory Practices. (c) Do not use reagents after the expiration date indicated on the label. (d) Visually inspect vials before testing. Do not use vials with evidence of damage, leakage, or deterioration. (e) Do not mix reagents (or disposables) from different lots. (f) Powder-free latex or nitrile gloves are recommended for all PCR steps. (g) The equipment and accessories should be regularly cleaned and decontaminated. (h) Never remove the caps from the lysis tubes. (i) Do not try to remove the strip tube caps once they have been sealed to the PCR strip tubes (j) Spills should be wiped up thoroughly after treatment with bleach or a nucleic acid degradation solution. See the GENE-UP® user manual for information on cleaning spills on or in the instrument. Do not autoclave solutions containing bleach. Listeria is a Gram ‑ positive, non ‑ spore ‑ forming, aerobic bacillus organism. Care must be taken when handling samples that may contain Listeria spp. Strict compliance with BSL ‑ 2 practices, containment equipment, and facilities are recommended for all activities utilizing known or potentially infectious clinical materials or cultures. While BSL ‑ 2 containment is suitable for all Listeria, BSL ‑ 3 practices and equipment are recommended for activities likely to produce significant aerosols or for activities involving production quantities of this particular organism. Laboratory personnel must be adequately trained to handle pathogens before being permitted to analyze samples for Listeria spp. Follow appropriate safety guidelines when handling potentially contaminated samples. Waste should be disposed of in compliance with local and national legislation. Listeriosis can be life ‑ threatening to certain vulnerable groups, such as pregnant women and those with compromised immune systems and can sometimes be life ‑ threatening to healthy individuals. Pregnant women and immune ‑ compromised individuals should take special care when handling samples that are potentially contaminated with Listeria. Safety Precautions