ERP Micro December 2019

AOAC Official Method 2019.xxx Listeria monocytogenes in a Variety of Foods and Select Environmental Samples: GENE-UP ® Listeria monocytogenes 2 (LMO 2) Test Method First Action 2019 Applicable to detection of Listeria monocytogenes in 25 g test portions of deli ham, deli roast beef, deli turkey, turkey hot dogs, fresh spinach, mixed bagged salad, cooked shrimp, smoked salmon, vanilla ice cream, Mexican soft cheese, pork rillettes, raw milk, salmon offcuts, mixed precooked vegetables, mixed salad and process water; 100 g test portions of whole liquid egg; 125 g test portions of deli ham & Mexican soft cheese; 375 g test portions of whey powder; stainless steel surface sponges

See Table 2019.1A for a summary of results of the inter-laboratory study. See Table 2019.2A for detailed results of the inter-laboratory study

A. Principle

The GENE-UP ® Listeria monocytogenes 2 (LMO 2) kit is designed for use with the GENE- UP ® Thermocycler. The GENE-UP ® LMO 2 kit contains all of the necessary components for PCR, including sample-specific primers and probes and an internal amplification control. The GENE-UP ® Thermocycler detects fluorescence at several wavelengths (channels) to allow for multi-target detection in the same reaction vessel. The fluorescent signal from a sample is recorded in channel 640, while the fluorescent signal for an internal amplification control is recorded in channel 705. The software automatically interprets the results for the internal amplification control and determines the sample result based on the outcome of the control. Both

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