ERP Micro December 2019

GENE-UP ® L. monocytogenes 2 (LMO 2)

050568 - 02 - 2018-12 - en

1 pouch with 1 control buffer vial

CTRL BUF Control buffer: 850 μL

Instructions for use are downloadable from www.biomerieux.com/techlib. REAGENTS, MATERIALS AND CONSUMABLES REQUIRED BUT NOT PROVIDED Equipment

Consumables

• GENE ‑ UP ® Thermocycler (REF 414056)

• GENE ‑ UP ® Lysis Kit (REF 414057)

Sample Manipulation

• LPT broth. For example (BIOMÉRIEUX REF): ◦ 6 x 225 mL (REF 410848) ◦ 6 x 100 mL (REF 410846) ◦ 20 x 10 mL (REF 410845) ◦ 4 x 3 liters (REF 410849) ◦ 10 x 225 mL mini ‑ bag (REF 410847)

• Blender with paddles. For example (BIOMÉRIEUX REF):

◦ SMASHER ® (REF AESAP1064) ◦ SMASHER ® XL (REF AESAP1100)

• Troemner vortex ‑ type mixer ◦ Advanced Digital Heavy-Duty Vortex Mixer 120 V (REF 9456TAHDUSA) ◦ Advanced Digital Heavy ‑ Duty Vortex Mixer 230 V (REF 9456TAHDEUA) Instead of the Troemner vortex ‑ type mixer, customers can use the Vortex ‑ Genie Pulse from Scientific Industries with the BIOMÉRIEUX GENE ‑ UP ® Lysis Rack Adaptor (REF 414570). Please contact BIOMÉRIEUX for additional information.

• Sterile filter blender bags. For example (BIOMÉRIEUX REF): ◦ Type P filter bag (REF AES400P/50G) ◦ SMASHER ® XL Bag 2L (REF 415180) ◦ SMASHER ® XL Bag 4L (REF 415181)

Pipettes

Confirmation

• Ottaviani Agosti Agar Plate (for example): ◦ ALOA ® 120 x 90 mm (REF AEB520079) ◦ ALOA ® 20 x 90 mm (REF AEB520080) • Fraser Broth (for example REF 42072) • API ® LIS (REF 10300)

• Adjustable, variable volume pipette ◦ 0.5–10 μL (single or multichannel pipette) ◦ Compatible with 10 μL and 20 μL

• RAPIDEC ® Lmono (REF 3700) • Fast Rhamnose (REF 415078)

Centrifugation

Pipette Tips

• Plate Centrifuge ◦ MPS1000 Mini PCR Plate Spinner 110 V (REF 419196) ◦ MPS1000 Mini PCR Plate Spinner 230 V (REF 414555) • GENE ‑ UP ® PCR Tube Holder (REF 414573) • GENE ‑ UP ® Lysis Tube Remover (REF 414469) • GENE ‑ UP ® Heavy Rack Holder (REF 414571) • GENE ‑ UP ® Lysis Tube Holder (REF 414572) • Troemner Vortex Mixer Adaptor (REF 421713)

• Compatible sterile, filter pipette tips for 20 μL

• 10 μL Biotix filter pipette tip (REF 419194)

GENE ‑ UP ® Accessories

WARNINGS AND PRECAUTIONS • For microbiological control only. • For professional use only. • The GENE ‑ UP ® L. monocytogenes 2 kit must be used with the GENE ‑ UP ® Lysis kit (REF 414057).

• Place the instrument in a room designed for microbiological analysis. • Comply with Good Laboratory Practice (e.g., standard EN ISO 7218). 7 • Do not use reagents after the expiration date indicated on the label.

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