ERP Micro December 2019

1600  B astin et al . : J ournal of AOAC I nternational V ol . 102, N o . 5, 2019

Table 2017.09B. Summary of results for the confirmation of Salmonella (inclusivity)

MALDI Biotyper correctly confirmed

Ref. correctly confirmed

MALDI Biotyper incorrectly confirmed

Ref. incorrectly confirmed

MALDI Biotyper not tested

Ref. not tested

MALDI Biotyper total

Ref. total

Organism a

Salmonella Cerro Ad1173 Salmonella Enteritidis Ad2539 Salmonella Newport Ad540 Salmonella Stourbridge Ad2297 Salmonella Agona A00V038 Salmonella Dublin Ad1336 Salmonella Panama Ad1733 Salmonella Typhimurium Ad1484 Salmonella Brandenburg Ad351 Salmonella Infantis Ad2646 Salmonella Napoli Ad928 Salmonella Saintpaul A00C002 Salmonella Derby Ad1337 Salmonella Kentucky Ad1756 Salmonella Virchow F276 Salmonella Montevideo Ad912

45 45 44 45 45 45 45 45 45 45 45 45 45 45 45 45

15 15 15 15 15 15 15 15 15 15 15 14 15 15 14 15

0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1

0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1

45 45 44 45 45 45 45 45 45 45 45 45 45 45 45 45

15 15 15 15 15 15 15 15 15 15 15 15 15 15 14 15

1 b

719 c

239 d

Total isolates

719

238

a  ADRIA Développement collection, Quimper, France. b  Not tested on RAPID’ Salmonella . c  The total numbers represent isolates analyzed on the three recommended culture media: Tryptic Soy Agar, Xylose Lysine Deoxycholate, and RAPID’ Salmonella . d  Reference method performed from the nonselective Tryptic Soy Agar plates only.

Table 2017.09C. Summary of results for the identification of Gram-negative organisms ( Salmonella exclusivity)

MALDI Biotyper incorrectly identified as Salmonella spp.

MALDI Biotyper correctly identified

Ref. correctly identified

Ref. incorrectly identified as Salmonella spp.

Other Ref. incorrectly identified

MALDI Biotyper not tested

MALDI Biotyper total

Ref. total

Organism a

Providencia stuartii Ad1575 Citrobacter braakii Ad2701 Cronobacter sakazakii Ad1418 Yersinia enterocolitica A00C066 Aeromonas hydrophila Ad1570 Escherichia hermanii Ad457 Plesiomonas shigelloides Ad673 Serratia marcescens Ad447

45 45 45 44 42 45 44 45

15 12 15 14 14

0 0 0 0 0 0 0 0 0

0 3 0 0 0 0 0 0 3

0 0 0 1 1

0 0 0 1 3 0 1 0 5

45 45 45 44 42 45 44 45

15 15 15 15 15 15 15 15

5

10

15 15

0 0

355 b

120 c

Total isolates

355

105

12

a  ADRIA Développement collection, Quimper, France. b  The total numbers represent isolates analyzed on the three recommended culture media: Tryptic Soy Agar, Xylose Lysine Deoxycholate, and RAPID’ Salmonella . c  Reference method performed from the nonselective Tryptic Soy Agar plates only.

current local, regional, and national standards for the disposal of contaminated waste. A. Principle The Bruker MALDI Biotyper ® Method is intended to be used for the automated identification and confirmation of bacteria. Classification and identifications are based on proteomic

use of chemicals and the need to perform certain steps in a chemical fume hood should also be evaluated. When testing is complete, all materials possibly containing pathogens should be decontaminated, following current industry standards for the disposal of contaminated waste (i.e., autoclave for 30 min at 121°C). Consult the Safety Data Sheet for additional information and local regulations for disposal. To reduce the risks associated with environmental contamination, follow